Oxidative damage by ozone and nitrogen dioxide: synergistic toxicity in vivo but no evidence of synergistic oxidative damage in an extracellular fluid.
Oxidative damage by ozone and nitrogen dioxide: synergistic toxicity in vivo but no evidence of synergistic oxidative damage in an extracellular fluid.
复制标题
臭氧和二氧化氮的氧化损伤：体内协同毒性，但没有证据表明细胞外液中存在协同氧化损伤。
DOI：
10.1042/bss0610139
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Cross,CE
中科院分区：
文献类型：
--
作者：
O'Neill,CA;vanderVliet,A;Eiserich,JP;Last,JA;Halliwell,B;Cross,CE
吸入臭氧（O3）和/或二氧化氮（.NO2）与呼吸道炎症的发展和肺功能的各种改变有关。呼吸道衬液（RTLF）代表了与这些吸入毒物接触的第一种生物液体。使用血浆作为RTLF的替代物，我们先前已经表明O3[Cross，Motchnik，Bruener，Jones，Kaur，艾姆斯和Halliwell（1992）FEBS Lett. 298，269-272]和NO2 [Halliwell，Hu，Louie，杜瓦尔，Tarkington，Motchnik和Cross（1992）FEBS Lett. 313，62-66]都能够消耗抗氧化剂和破坏蛋白质和脂质。O3特别损害蛋白质，而NO2诱导脂质过氧化和硝酸盐芳香族氨基酸。据报道，O3和NO2在啮齿动物中引起协同毒性[Gielzleichter，Witschi和Last（1992）Tox.应用药理学116，1-9]。在本章中，我们回顾的证据表明，这两种氧化剂气体的组合暴露于人体血浆不能发挥协同氧化损伤的血浆成分，事实上，O3和。NO2拮抗对方的行动。我们的结论是，这两种气体对啮齿动物的发病率和死亡率的增强作用代表了一个复杂的相互作用的生物效应，而不是一个简单的协同作用在细胞外液中的氧化作用。
Inhalation of ozone (O3) and/or nitrogen dioxide (.NO2) is associated with the development of inflammation in the respiratory tract and various alterations in pulmonary functions. Respiratory tract lining fluids (RTLFs) represent the first biological fluids coming into contact with these inhaled toxicants. Using plasma as a surrogate for RTLFs, we have previously shown that O3[Cross, Motchnik, Bruener, Jones, Kaur, Ames and Halliwell (1992) FEBS Lett. 298, 269-272] and.NO2[Halliwell, Hu, Louie, Duvall, Tarkington, Motchnik and Cross (1992) FEBS Lett. 313, 62-66] are both capable of depleting antioxidants and damaging proteins and lipids. O3particularly damages proteins, whereas.NO2induces the peroxidation of lipids and nitrates aromatic amino acids. It has been reported that O3and.NO2cause synergistic toxicity in rodents [Gielzleichter, Witschi and Last (1992) Tox. Appl. Pharmacol. 116, 1-9]. In the present chapter, we review evidence showing that combined exposure of these two oxidant gases to human plasma fails to exert synergistic oxidative damage to plasma constituents, and in fact, O3and.NO2antagonize each other's actions. We conclude that the potentiating effect of these two gases on morbidity and mortality in rodents represents a complex interactive biological effect rather than a simple synergistic oxidative effect in extracellular fluids.