An Epstein-Barr virus transcript from a latently infected, growth-transformed B-cell line encodes a highly repetitive polypeptide.
An Epstein-Barr virus transcript from a latently infected, growth-transformed B-cell line encodes a highly repetitive polypeptide.
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来自潜伏感染、生长转化的 B 细胞系的 Epstein-Barr 病毒转录物编码高度重复的多肽。
DOI：
10.1073/pnas.83.24.9298
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发表时间：
1986
影响因子：
11.1
通讯作者：
Strominger,JL
中科院分区：
文献类型：
--
作者：
Speck,SH;Pfitzner,A;Strominger,JL
通过筛选从Epstein-Barr病毒潜伏感染的生长转化的人B淋巴母细胞系分离的poly（A）+ RNA制备的cDNA文库，我们已经回收了对应于主要由主要内部重复序列（IR 1）编码的高度剪接的病毒转录物的克隆。5'区含有一个拷贝的26碱基对（bp）外显子（W 0）[其位于CAATT-（N）34 TATAAA序列（N，未指定碱基）下游28 bp处]和七个拷贝的两个小外显子（W1，66 bp; W2，132 bp）。此外，有三个外显子来自病毒基因组的BamHI Y片段的“独特”区域。已经描述的另外两个cDNA克隆，对应于潜伏病毒转录物，在它们的5'区与该克隆共享同源性，并且在它们的3'端明显不同。本文描述的cDNA克隆包含一个延伸穿过重复元件的长开放阅读框。该开放阅读框的体外转录和翻译产生了一种62 kDa的多肽，该多肽可以被EB病毒阳性人血清免疫沉淀。
By screening a cDNA library prepared from poly(A)+ RNA isolated from an Epstein-Barr virus latently infected, growth-transformed human B-lymphoblastoid cell line, we have recovered a clone corresponding to a highly spliced viral transcript encoded largely by the major internal repeat (IR1). The 5' region contains one copy of a 26-base-pair (bp) exon (W0) [which is 28 bp downstream from a CAATT-(N)34TATAAA sequence (N, unspecified base)] and seven copies of two small exons (W1, 66 bp; W2, 132 bp). In addition, there are three exons from the "unique" region of the BamHI Y fragment of the viral genome. Two other cDNA clones that have been described, corresponding to latent viral transcripts, share homology in their 5' regions with this clone and are clearly divergent at their 3' ends. The cDNA clone described in this paper contains one long open reading frame that extends through the repeat element. In vitro transcription and translation of this open reading frame yielded a 62-kDa polypeptide that could be immunoprecipitated by an Epstein-Barr virus-positive human serum.