Photodynamic treatment of red blood cell concentrates for virus inactivation enhances red blood cell aggregation: Protection with antioxidants
Photodynamic treatment of red blood cell concentrates for virus inactivation enhances red blood cell aggregation: Protection with antioxidants
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DOI：
10.1111/j.1751-1097.1997.tb03181.x
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发表时间：
1997-10-01
影响因子：
3.3
通讯作者：
Yedgar, S
中科院分区：
文献类型：
--
作者：
BenHur, E;Barshtein, G;Yedgar, S
使用酞菁和红光的光动力学处理(PDT)似乎是一种很有前途的去污染红细胞(RBC)浓缩输血的方法。这种治疗的一个可能的并发症可能是诱导红细胞聚集。用一种新型的计算机化细胞特性分析仪(CFA)测量红细胞聚集物的产生。磺化铝酞菁(AlPcS4)和含硅酞菁Pc4的RBC浓缩物在杀毒条件下的PDT显著促进了RBC聚集，分散这些聚集体需要较高的切应力。与未经处理的红细胞形成的圆形不同，细胞簇巨大且形状异常，当在光动力疗法中加入抗氧化剂混合物时，这种聚集被阻止。在PDT后加入抗氧化剂只能部分减少聚集。结论是，在输血前对RBC浓缩物进行PDT期间加入抗氧化剂可能会减少或消除抗病毒治疗可能给接受者带来的血流动力学风险。
Photodynamic treatment (PDT) using phthalocyanines and red light appears to be a promising procedure for decontamination of red blood cell (RBC) concentrates for transfusion. A possible complication of this treatment may be induced aggregation of RBC. The production of RBC aggregates was measured with a novel computerized cell how properties analyzer (CFA). The PDT of RBC concentrates with sulfonated aluminum phthalocyanine (AlPcS4) and the silicon phthalocyanine Pc 4 under virucidal conditions markedly enhanced RBC aggregation and higher shear stress was required to disperse these aggregates. The clusters of cells were huge and abnormally shaped, unlike the rouleaux formed by untreated RBC, This aggregation was prevented when a mixture of antioxidants was included during PDT. Addition of the antioxidants after PDT reduced aggregation only partially. It is concluded that inclusion of antioxidants during PDT of RBC concentrates prior to transfusion may reduce or eliminate the hemodynamic risk that the virucidal treatment may present to the recipient.