The development of rheometry for strain-sensitive gelling systems and its application in a study of fibrin-thrombin gel formation
The development of rheometry for strain-sensitive gelling systems and its application in a study of fibrin-thrombin gel formation
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应变敏感凝胶体系流变测定法的发展及其在纤维蛋白-凝血酶凝胶形成研究中的应用
DOI：
10.1007/s00397-010-0473-6
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发表时间：
2010
期刊：
影响因子：
2.3
通讯作者：
Hawkins K
中科院分区：
文献类型：
--
作者：
Hawkins K
本文报道了由傅里叶变换力学谱获得的时间分辨粘弹性数据重建的模拟序列频率扫描数据。结果表明，在“快速”成胶条件下，凝胶点应力松弛幂律指数α的异常值可能是由于流变技术不当造成的。建立了一个适当的流变学标准，用于连续频率扫描的应用，以获得准确的α值，在应变敏感，快速形成凝胶的形成。此外，使用适当的流变仪，我们报告的α值的纤维蛋白-凝血酶凝胶形成的凝血酶添加到生理相关水平的人纤维蛋白原，并将这些值与纤维蛋白凝胶网络的微观结构的分形维数。本研究是第一个报告的修改的分形特性的初始凝块在纤维蛋白-凝血酶凝胶由于凝血酶的可用性。这项工作证实了以下假设：在血液样本的凝胶点处记录的自相似（分形）应力松弛行为（Evans等人，2010 a，B）与初始血凝块纤维蛋白网络的微观结构特征相关。
This paper reports simulated sequential frequency sweep data which have been reconstructed from time resolved viscoelastic data obtained by Fourier transform mechanical spectroscopy. Comparisons of the results show that the recording of anomalous values of the stress relaxation power law exponentαat the Gel Point under ‘rapid’ gelling conditions may be due to inappropriate rheological techniques. An appropriate rheometrical criterion is established for the application of sequential frequency sweeps in order to obtain accurate values ofαin the formation of strain-sensitive, rapidly formed gels. Furthermore, using appropriate rheometry, we report values ofαfor fibrin–thrombin gels formed by the addition of thrombin to a physiologically relevant level of human fibrinogen, and relate these values to the microstructure of the fibrin gel network in terms of a fractal dimension. The present study is the first to report a modification of the fractal characteristics of incipient clots in fibrin–thrombin gels due to the availability of thrombin. This work confirms the hypothesis that the self-similar (fractal) stress relaxation behaviour recorded at the Gel Point of samples of coagulating blood (Evans et al. 2010a, b) is associated with the microstructural characteristics of the incipient blood clot’s fibrin network.