CDMP-2 injection improves early tendon healing in a rabbit model for surgical repair
CDMP-2 injection improves early tendon healing in a rabbit model for surgical repair
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DOI：
10.1111/j.1600-0838.2005.00462.x
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发表时间：
2005-08-01
影响因子：
4.1
通讯作者：
Aspenberg, P
中科院分区：
文献类型：
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作者：
Virchenko, O;Fahlgren, A;Aspenberg, P
本研究验证了软骨衍生形态发生蛋白-2(CDMP-2)可以促进肌腱手术修复后愈合的假说。我们之前已经发现，在大鼠和兔的机械性跟腱缺损保守治疗模型中，应用CDMP-2可以改善肌腱愈合。本研究以40只兔为实验对象，用派特罗-胫骨环扎术剥离并横切髌腱。术后2小时，血肿内注射CDMP-2或赋形剂20 mg。分别于第14天和第28天取材，进行生物力学测试、放射学和组织学评价。在第14天，与对照组相比，CDMP-2使破坏时的力增加了65%，极限应力增加了50%，刚度增加了57%。对愈伤组织的大小没有影响。在28天时，治疗组之间没有表现出差异。在任何时间点的肌腱或再生组织中均未发现骨或软骨。因此，CDMP-2可以在无负荷模型中刺激肌腱的早期修复。这些结果提示CDMP-2可能作为肌腱断裂手术治疗的补充在临床上有意义。
This study examines the hypothesis that cartilage-derived morphogenic protein-2 (CDMP-2) can improve tendon healing after surgical repair. We have previously found improved tendon healing by applying CDMP-2 in models for conservative treatment with mechanically loaded Achilles tendon defects in rats and rabbits. In this study, the patellar tendon was unloaded by patello- tibial cerclage and cut transversely in 40 rabbits. Two hours post-operative, the rabbits received a dose of 20 mu g of CDMP-2 or vehicle injected into the hematoma. Specimens were harvested after 14 and 28 days and evaluated by biomechanical testing, radiography and histology. At 14 days, CDMP-2 caused a 65% increase in force at failure, a 50% increase in ultimate stress and a 57% increase in stiffness, as compared with controls. There was no effect on callus size. At 28 days, no differences between the treatment groups could be demonstrated. No bone or cartilage was found in any tendon or regenerated tissue at any time point. Thus, early tendon repair can be stimulated by CDMP-2 in an unloaded model. These results suggest that CDMP-2 might be of interest for clinical use as a complement to surgical treatment of tendon ruptures.