Identification of transcription factor ZmZAT8 involved in abscisic acid regulation pathway of starch synthesis in maize endosperm
Identification of transcription factor ZmZAT8 involved in abscisic acid regulation pathway of starch synthesis in maize endosperm
复制标题
玉米胚乳淀粉合成脱落酸调节途径转录因子ZMZAT8的鉴定
DOI：
10.30848/pjb2019-6(26
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发表时间：
2019-06
影响因子：
1.2
通讯作者：
Zhang Junjie
中科院分区：
文献类型：
--
作者：
Jiang Tinglan;Xia Miao;Huang Huanhuan;Xiao Jianlu;Long Jiao;Li Xiao;Zhang Junjie
玉米淀粉具有广泛的用途，它与玉米的产量和品质密切相关。尽管淀粉生物合成的主要途径已经确定，但涉及的全部基因及其表达调控机制仍不清楚。在本研究中，根据我们先前发表的阿坝处理玉米胚乳的转录组数据，选择并克隆了一个新的基因，命名为ZmZAT 8作为候选基因。生物信息学分析表明，ZmZAT 8编码21.6kD的蛋白，包含184个氨基酸和两个QALGGH结构域，是植物C2 H2型锌指蛋白的保守特征。实时荧光定量RT-PCR分析证实，阿坝能强烈刺激ZmZAT 8基因的表达。此外，对ZmZAT 8进行了一系列评价，包括亚细胞定位、转录激活、蛋白质-DNA相互作用和在玉米胚乳中的瞬时表达的分析。结果表明，ZmZAT 8蛋白能够调控淀粉合成关键基因Sh 2、Bt 1和Wx的表达。总之，我们的数据表明，ZmZAT 8，一个新的ABA诱导的C2 H2型锌指转录因子，可能在玉米胚乳淀粉合成中发挥积极的作用。
Maize starch, which is closely related with the yield and the quality of the produced maize, has wide applications. Even though the main pathway of starch biosynthesis has been identified, the full array of genes involved and the mechanisms regulating their expression are still unclear. In this study, a new gene, named ZmZAT8, was chosen and cloned as a candidate gene based on our previously published transcriptome data of maize endosperm treated with ABA. Bioinformatics analysis indicated that ZmZAT8 encodes 21.6kD protein comprising 184 amino acids and two QALGGH domains, which is a conserved feature of plant C2H2-type Zinc-finger proteins. ABA could strongly stimulate the expression of ZmZAT8, as confirmed by real-time RT-PCR analysis. Moreover, a series of evaluations regarding the ZmZAT8 were performed, including analyses of subcellular localization, transcription activation, protein-DNA interactions, and transient expression in maize endosperm. The results showed that the ZmZAT8 protein was capable of regulating the expression of Sh2, Bt1, and Wx, which were all key genes involved in starch synthesis. In conclusion, our data suggests that ZmZAT8, a novel ABA-induced C2H2-type zinc-finger transcription factor, may play a positive role in regulating starch synthesis in maize endosperm.
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影响因子：
7.4
作者：
Hennen-Bierwagen, Tracie A.;Lin, Qiaohui;Myers, Alan M.
通讯作者：
Myers, Alan M.
蔗糖和脱落酸对玉米胚乳淀粉合成相关基因的协同影响
影响因子：
3.1
作者：
Chen, Jiang;Huang, Binquan;Huang, Yubi
通讯作者：
Huang, Yubi
ABI4 与 CACCG 基序的结合介导 ABA 诱导的玉米 (Zea mays L.) 胚乳中 ZmSSI 基因的表达
影响因子：
6.9
作者：
Hu, Yu-Feng;Li, Yang-Ping;Huang, Yubi
通讯作者：
Huang, Yubi
DOI：
10.1146/annurev.pp.39.060188.002255
发表时间：
1988
期刊：
--
影响因子：
--
作者：
J. Zeevaart
通讯作者：
J. Zeevaart
影响因子：
27.5
作者：
Li, Tian;Wu, Xiu-Yun;Liu, Jin-Yuan
通讯作者：
Liu, Jin-Yuan