Expression of TASK-1 in brainstem and the occurrence of central sleep apnea in rats
Expression of TASK-1 in brainstem and the occurrence of central sleep apnea in rats
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TASK-1在大鼠脑干中的表达与中枢性睡眠呼吸暂停的发生
DOI：
10.1016/j.resp.2007.11.008
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发表时间：
2008-03-20
影响因子：
2.3
通讯作者：
Wang, GuangFa
中科院分区：
文献类型：
--
作者：
Wang, Jing;Zhang, Cheng;Wang, GuangFa
近年来的研究表明，通气控制不稳定是睡眠呼吸暂停发生的机制之一。因此，我们研究了酸敏感性钾通道Task-I是否在睡眠呼吸暂停的发生中起作用。首先，用原位杂交技术检测脑干TASK-I转录因子的表达。然后，从27只雄性大鼠中分析中枢性呼吸暂停发作与通过western blot测量的ASK-1蛋白含量之间的相关性。结果表明，在蓝斑、孤束核和延髓中缝等中枢化学感受器上广泛分布有ASK-1 mRNA，总自发性呼吸暂停指数（TSAI）和NREM睡眠中自发性呼吸暂停指数（NSAI）与ASK-1蛋白含量均呈正相关（r分别为0.547和0.601，P < 0.01）。
Recent studies revealed that unstable ventilation control is one of mechanisms underlying the occurrence of sleep apnea. Thus, we investigated whether TASK-1, an acid-sensitive potassium channel, plays a role in the occurrence of sleep apnea. First, the expression of TASK-I transcriptions on brainstem was checked by in situ hybridization. Then, the correlation between the central apneic episodes and protein contents of TASK-1 measured by western blot was analyzed from 27 male rats. Results showed that TASK-1 mRNAs were widely distributed on the putative central chemoreceptors such as locus coeruleus, nucleus tractus solitarius and medullary raphe, etc. Both the total spontaneous apnea index (TSAI) and spontaneous apnea index in NREM sleep (NSAI) were positively correlated with TASK-1 protein contents (r=0.547 and 0.601, respectively, p