MIR166/165 genes exhibit dynamic expression patterns in regulating shoot apical meristem and floral development in Arabidopsis
MIR166/165 genes exhibit dynamic expression patterns in regulating shoot apical meristem and floral development in Arabidopsis
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DOI：
10.1007/s00425-006-0439-1
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发表时间：
2007-05-01
期刊：
影响因子：
4.3
通讯作者：
Park, Chung-Mo
中科院分区：
文献类型：
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作者：
Jung, Jae-Hoon;Park, Chung-Mo
miR166/165组及其靶基因调控植物发育的多个方面，包括顶端和外侧分生组织形成、叶片极性和维管发育。本研究表明，MIR166/165基因与WUSCHEL (WUS)-CLAVATA （CLV）通路平行，在动态调控茎尖分生组织（SAM）和花发育。虽然miR166和miR165切割相同的靶mrna，但单个miR166 /165基因在不同植物组织中表现出不同的表达域。MIR166/165的表达也被暂时调控。与动态表达模式一致的是，在mir166 /165高产植株中，SAM活性和花结构发生了一系列变化。此外，当mir166a过表达突变体与WUS-CLV通路上的缺陷突变体进行遗传杂交时，所产生的杂交表现出加性表型效应，这表明mir166 /165介导的信号通过不同的信号通路发挥作用。
The miR166/165 group and its target genes regulate diverse aspects of plant development, including apical and lateral meristem formation, leaf polarity, and vascular development. We demonstrate here that MIR166/165 genes are dynamically controlled in regulating shoot apical meristem (SAM) and floral development in parallel to the WUSCHEL (WUS)-CLAVATA (CLV) pathway. Although miR166 and miR165 cleave same target mRNAs, individual MIR166/165 genes exhibit distinct expression domains in different plant tissues. The MIR166/165 expression is also temporarily regulated. Consistent with the dynamic expression patterns, an array of alterations in SAM activities and floral architectures was observed in the miR166/165-overproducing plants. In addition, when a MIR166a-overexpressing mutant was genetically crossed with mutants defective in the WUS-CLV pathway, the resultant crosses exhibited additive phenotypic effects, suggesting that the miR166/165-mediated signal exerts its role via a distinct signaling pathway.