ReCombine: a suite of programs for detection and analysis of meiotic recombination in whole-genome datasets.
ReCombine: a suite of programs for detection and analysis of meiotic recombination in whole-genome datasets.
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DOI：
10.1371/journal.pone.0025509
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Fung JC
中科院分区：
文献类型：
--
作者：
Anderson CM;Chen SY;Dimon MT;Oke A;DeRisi JL;Fung JC
在减数分裂过程中，染色体之间通过同源重组进行DNA交换是确保染色体正确分离和增加遗传多样性的关键步骤。重组的产品包括互惠交换，也就是所谓的交叉，以及非互惠的基因转换或非交叉。减数分裂重组的机制仍然不清楚，这在很大程度上是因为用传统的遗传学方法分析大量重组事件的难度。这些传统方法正越来越多地被能够在全基因组基础上调查减数分裂重组的高通量技术所取代。下一代测序或微阵列杂交被用来在杂交酵母菌株的后代中对数千个多态标记进行基因分型。需要新的计算工具来进行这种基因分型，并发现和分析重组事件。我们已经开发了一套程序，重组，用于使用来自下一代测序实验的短序列读取来对酵母减数分裂后代进行基因分型。在基因分型时，程序Crossver是重组的一个组成部分，然后检测重组产物，并根据在每个位置发现的特征及其在不同染色单体中的分布将它们分类。Crosover还能够分析来自微阵列实验或其他来源的分离数据。这套程序旨在允许即使是没有计算专业知识的研究人员也可以使用高通量的全基因组方法来研究减数分裂重组的分子机制。
In meiosis, the exchange of DNA between chromosomes by homologous recombination is a critical step that ensures proper chromosome segregation and increases genetic diversity. Products of recombination include reciprocal exchanges, known as crossovers, and non-reciprocal gene conversions or non-crossovers. The mechanisms underlying meiotic recombination remain elusive, largely because of the difficulty of analyzing large numbers of recombination events by traditional genetic methods. These traditional methods are increasingly being superseded by high-throughput techniques capable of surveying meiotic recombination on a genome-wide basis. Next-generation sequencing or microarray hybridization is used to genotype thousands of polymorphic markers in the progeny of hybrid yeast strains. New computational tools are needed to perform this genotyping and to find and analyze recombination events. We have developed a suite of programs, ReCombine, for using short sequence reads from next-generation sequencing experiments to genotype yeast meiotic progeny. Upon genotyping, the program CrossOver, a component of ReCombine, then detects recombination products and classifies them into categories based on the features found at each location and their distribution among the various chromatids. CrossOver is also capable of analyzing segregation data from microarray experiments or other sources. This package of programs is designed to allow even researchers without computational expertise to use high-throughput, whole-genome methods to study the molecular mechanisms of meiotic recombination.
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酵母重组过程中减数分裂后分离的全基因组调查。
影响因子：
12.3
作者：
Mancera E;Bourgon R;Huber W;Steinmetz LM
通讯作者：
Steinmetz LM
影响因子：
64.5
作者：
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影响因子：
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作者：
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通讯作者：
Durbin, Richard
影响因子：
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作者：
Mancera, Eugenio;Bourgon, Richard;Brozzi, Alessandro;Huber, Wolfgang;Steinmetz, Lars M.
通讯作者：
Steinmetz, Lars M.
影响因子：
7
作者：
Ning, ZM;Cox, AJ;Mullikin, JC
通讯作者：
Mullikin, JC