Fluorescence microendoscopy imaging based on GRIN lenses with one- and two-photon excitation modes
Fluorescence microendoscopy imaging based on GRIN lenses with one- and two-photon excitation modes
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基于单光子和双光子激发模式GRIN透镜的荧光显微内窥镜成像
DOI：
10.1007/s12200-015-0503-1
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发表时间：
2015-06-01
影响因子：
5.4
通讯作者：
Niu, Hanben
中科院分区：
文献类型：
--
作者：
Yan, Wei;Peng, Xiao;Niu, Hanben
随着生命科学的快速发展，对小动物活体荧光成像的需求越来越大。然而，在传统的荧光显微镜物镜的大尺寸和有限的工作距离限制了其成像应用主要是浅表组织。为了克服这些缺点，研究人员已经开发出具有小直径的梯度折射率（GRIN）探头，用于以微创方式对小动物的内部器官进行成像。然而，基于梯度折射率透镜的动态成像还没有得到广泛的研究。本文提出了一种基于梯度折射率透镜的单光子和双光子荧光内窥成像系统。系统中使用了直径为1.15 mm、长度为7.65 mm的梯度折射率透镜。通过紧凑的激光扫描成像系统获得图像，该系统具有用于扫描激光束的谐振振镜系统和用于检测荧光信号的光电倍增管（PMT）。 这将是一个有用的工具，研究小动物或植物在体内的生物过程。
With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in traditional fluorescence microscopes have limited their imaging applications mostly to superficial tissues. To overcome these disadvantages, researchers have developed the graded-index (GRIN) probes with small diameters for imaging internal organs of small animals in a minimally invasive fashion. However, dynamic imaging based on GRIN lens has not been studied extensively. Here, this paper presented a fluorescence endoscopic imaging system based on GRIN lenses using one-photon and two-photon excitation. GRIN lenses with 1.15 mm diameter and 7.65 mm length were used in the system. The images were acquired by a compact laser scanning imaging system with a resonant galvo-mirror system to scan the laser beam and a photomultiplier tube (PMT) to detect fluorescence signals. It would be a useful tool for studying biological processes of small animals or plants in vivo.