DNA TECHNIQUES APPLIED TO THE IDENTIFICATION OF PINCTADA FUCATA PEARLS FROM UWAJIMA, EHIME PREFECTURE, JAPAN
DNA TECHNIQUES APPLIED TO THE IDENTIFICATION OF PINCTADA FUCATA PEARLS FROM UWAJIMA, EHIME PREFECTURE, JAPAN
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DOI：
10.5741/gems.54.1.40
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发表时间：
2018-03-01
期刊：
影响因子：
2.6
通讯作者：
Sturman, Nicholas
中科院分区：
文献类型：
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作者：
Saruwatari, Kazuko;Suzuki, Michio;Sturman, Nicholas
遗传物质脱氧核糖核酸（DNA）是鉴定珍珠品种的有用指标，前提是可以从足够小的珍珠粉中提取和扩增目标成分，从而使该技术基本上是无损的。本研究以Meyer等人（2013）的类似工作为基础，但重点关注日本爱媛县乌瓦吉马的akoya养殖珍珠。在这里，我们还靶向16S rRNA基因，DNA的线粒体组分编码16S rRNA（核糖体核糖核酸）。我们成功地从供体和宿主珍珠牡蛎的外套膜组织和510 mg珍珠粉样品中扩增了16S rRNA基因。结果表明与合浦珠母贝16S rRNA基因相匹配。
The genetic material deoxyribonucleic acid (DNA) is a useful indicator in identifying pearl species, provided the extraction and amplification of a targeted component is possible from amounts of pearl powder small enough to render the technique essentially nondestructive. This study builds on similar work carried out by Meyer et al. (2013) but focuses on akoya cultured pearls from Uwajima in Ehime Prefecture, Japan. Here, we also target the 16S rRNA genes, the mitochondrial component of DNA to code 16S rRNA (ribosomal ribonucleic acid). We successfully amplified the 16S rRNA gene from the mantle tissues of donor and host pearl oysters and from 510 mg of pearl powder samples. The results indicate a match with the Pinctada fucata 16S rRNA gene.