Detection of aneuploidy by array comparative genomic hybridization using cell lines to mimic a mosaic trophectoderm biopsy
Detection of aneuploidy by array comparative genomic hybridization using cell lines to mimic a mosaic trophectoderm biopsy
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DOI：
10.1016/j.fertnstert.2011.12.048
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发表时间：
2012-04-01
影响因子：
6.7
通讯作者：
SenGupta, Sioban
中科院分区：
文献类型：
--
作者：
Mamas, Thalia;Gordon, Anthony;SenGupta, Sioban
设计：实验研究地点：大学实验室。材料(S)：上皮细胞系。干预(S)：将整倍体和非整倍体细胞混合建立马赛克滋养外胚层和囊胚模型。主要观察指标(S)：阵列比较基因组杂交后测量不同比例的非整倍体细胞的非整倍体水平。结果(S)：当样本中的非整倍体细胞水平为25%时，出现正常的转变。当非整倍体细胞水平为50%时，可以肯定地称为非整倍体。结论：本研究确定了阵列比较基因组杂交可以检测到马赛克样品中的非整倍体，其结果也可能表明样品中存在非整倍体细胞的比例。(Fertil Steril(R)2012；97：943-7.(C)2012年，由美国生殖医学学会主办。)
Objective: To examine the effect of mosaicism in the array comparative genomic hybridization result during preimplantation genetic screening after blastocyst biopsy.Design: Experimental study.Setting: University laboratory.Material(s): Epithelial cell lines.Intervention(s): Mixing of euploid and aneuploid cells to create mosaic trophectoderm and blastocyst models.Main Outcome Measure(s): The level of aneuploidy in samples with different ratios of aneuploid cells was measured after array comparative genomic hybridization.Result(s): A shift from normality was present when the level of aneuploid cells in the sample was >25%. Aneuploidy could be confidently called when the level of aneuploid cells was >50%.Conclusion(s): This study determined that aneuploidy in mosaic samples can be detected by array comparative genomic hybridization and that the result may also indicate the proportion of the aneuploid cells present in the sample. (Fertil Steril (R) 2012; 97: 943-7. (C) 2012 by American Society for Reproductive Medicine.)