Semi-hydrolysate of paper pulp without pretreatment enables a consolidated fermentation system with in situ product recovery for the production of butanol.
Semi-hydrolysate of paper pulp without pretreatment enables a consolidated fermentation system with in situ product recovery for the production of butanol.
复制标题
DOI：
10.1016/j.biortech.2019.01.043
复制
发表时间：
2019-04
影响因子：
11.4
通讯作者：
Tao Zhao;K. Yasuda;Y. Tashiro;Rizki Fitria Darmayanti;K. Sakai;K. Sonomoto
中科院分区：
文献类型：
--
作者：
Tao Zhao;K. Yasuda;Y. Tashiro;Rizki Fitria Darmayanti;K. Sakai;K. Sonomoto
利用木质纤维素生物质进行生物丁醇发酵通常需要昂贵的预处理和酶水解过程。在这项研究中，纸浆（93.2%葡聚糖）被用作生物质原料生产生物丁醇。我们在没有预处理和低酶负载的情况下对纸浆进行了酶促半水解，产生了高浓度的纤维二糖（13.9 g L−1）和葡萄糖（21.3 g L−1）。此外，半水解产物的有效发酵与使用商业糖而不使用抑制剂类似。最后，设计了一种新型的非等温同步糖化发酵-丁醇回收工艺，该工艺由重复半水解过程和连续丁醇萃取发酵过程组成。与其他整合的水解和发酵工艺相比，合并的系统提高了丁醇产量、丁醇产率和丁醇生产率，并且能够重复使用培养基。
Utilization of lignocellulosic biomasses for biobutanol fermentation usually requires costly processes of pretreatment and enzymatic hydrolysis. In this study, paper pulp (93.2% glucan) was used as a starting biomass material to produce biobutanol. We conducted enzymatic semi-hydrolysis of paper pulp without pretreatment and with low enzyme loading, which produced high concentrations of cellobiose (13.9 g L−1) and glucose (21.3 g L−1). In addition, efficient fermentation of the semi-hydrolysate was achieved similar to that with the use of commercial sugars without inhibitors. Finally, we designed a novel non-isothermal simultaneous saccharification and fermentation within situbutanol recovery, which was composed of a repeated semi-hydrolysis process and successive butanol-extractive fermentation process under the respective optimal conditions. The consolidated system improved butanol production, butanol yields, and butanol productivities and enabled repeated use of medium when compared with other integrated hydrolysis and fermentation processes.