Surfactant stimulation of growth in the nematode Caenorhabditis elegans
Surfactant stimulation of growth in the nematode Caenorhabditis elegans
复制标题
DOI：
10.1016/s0141-0229(96)01173-8
复制
发表时间：
1997-05-01
影响因子：
3.4
通讯作者：
dePomerai, DI
中科院分区：
文献类型：
--
作者：
Mutwakil, MHAZ;Steele, TJG;dePomerai, DI
尺寸分级已用于从线虫秀丽隐杆线虫的混合培养物中分离 L1/L2 幼虫。比较了在同步液体和琼脂培养物中生长过程中的蠕虫长度。在液体 S 培养基中添加 10 ppm 表面活性剂（Pluronics F-68、F-127、F-38、L-35；Tween-20 或 Triton X-100）在所有情况下均促进了三种粘土的生长显着刺激。由于液体培养物中可能营养不良和/或通气不良，因此在标准 NGM 琼脂中使用相同的表面活性剂重复实验。四种表面活性剂再次显着刺激蠕虫生长，而两种（Tween-20 和 Pluronic F-68）则没有。这些表面活性剂之间的差异还体现在：（i）毒性； (ii) 在转基因热休克蛋白/报告菌株中诱导应激反应； (iii) 在表面活性剂处理开始时和 41 小时后刺激氨基酸掺入可溶性蛋白质。这些表面活性剂，特别是 Pluronic F-127，作为促进线虫幼虫生长的培养补充剂具有潜在价值。根据固定的体细胞谱系和 Pluronic F-127 没有加速从 L4 幼虫到产卵成虫的成熟这一事实，讨论了表面活性剂促进生长的可能机制。 (C) 1997 年，爱思唯尔科学公司。
Size fractionation has been used to isolate L1/L2 larvae from mixed cultures of the nematode, Caenorhabditis elegans. Worm lengths have been compared during growth in synchronized liquid and agar cultures. Supplementation of liquid S medium with 10 ppm surfactant (Pluronics F-68, F-127, F-38, L-35; Tween-20 or Triton X-100) promoted a significant stimulation of growth over three clays in all cases. Because of possible poor nutrition and/or aeration in liquid culture, experiments were repeated using the same surfactants in standard NGM agar. Four surfactants again stimulated worm growth significantly whereas two (Tween-20 and Pluronic F-68) did not. Differences between these surfactants were also demonstrated with respect to: (i) toxicity; (ii) induction of stress responses in a transgenic hsp/reporter strain; and (iii) stimulation of amino acid incorporation into soluble protein both initially and after 41 h of surfactant treatment. These surfactants, and in particular, Pluronic F-127, are potentially valuable as culture supplements for enhancing nematode larval growth. Possible mechanisms for growth promotion by surfactants are discussed in light of the fixed somatic cell lineage and the fact that Pluronic F-127 did not speed up maturation from L4 larvae into egg-bearing adults. (C) 1997 by Elsevier Science Inc.