Immunoproteomics of extracellular proteins of Chinese virulent strains of Streptococcus suis type 2
Immunoproteomics of extracellular proteins of Chinese virulent strains of Streptococcus suis type 2
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DOI：
10.1002/pmic.200700294
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发表时间：
2007-12-01
期刊：
影响因子：
3.4
通讯作者：
Lu, Cheng Ping
中科院分区：
文献类型：
--
作者：
Zhang, Wei;Lu, Cheng Ping
猪链球菌2型（SS2）是一种分布在世界各地的猪人畜共患病原体，缺乏合适的疫苗和毒力制造者是控制其感染的瓶颈。采用免疫蛋白组学方法从中国SS2毒株ZY05719的细胞外总蛋白中鉴定抗原蛋白。SPF迷你猪恢复期血清可识别PVDF膜上的9个蛋白斑点。复制凝胶上的抗原蛋白，以及2d凝胶上另一株毒力菌株（HA9801）和一株无毒菌株（T15）的细胞外蛋白的相似放置，被切除并通过MALDI-TOF-MS鉴定。利用MASCOT服务器对蛋白斑点进行PMF检测。在这三种菌株中都发现了两种蛋白质。两株毒株与无毒株的蛋白质组学比较分析发现了9个差异蛋白，其中8个成功鉴定。在两种毒性菌株中发现了6种差异表达蛋白的基因，其中6种差异表达蛋白存在于无毒菌株中。
Streptococcus suis type 2 (SS2) is a porcine zoonotic pathogen with worldwide distribution, and lacking suitable vaccine and virulent maker were bottleneck to control this infection. An immunoproteomic assay was used to identify antigenic proteins from the total extracellular proteins of the virulent Chinese SS2 strain ZY05719. The convalescent serum of a specific pathogen free (SPF) mini-pig recognized nine protein spots on PVDF membrane. Antigenic proteins on a duplicate gel, as well as those with a similar placement of extracellvdar proteins from another virulent strain (HA9801) and an avirulent strain (T15) on 2-D gels, were excised and identified by MALDI-TOF-MS. PMF of the protein spots were performed using the MASCOT server. Two proteins were found in all three strains. Comparative proteomic analysis between the two virulent strains and the avirulent strain revealed nine differential proteins, eight of which were successfully identified. Genes for six of the differentially expressed proteins were found in both virulent strains, and of those were present in the avirulent stain.