Composition of supralittoral sediments bacterial communities in a Mediterranean island
Composition of supralittoral sediments bacterial communities in a Mediterranean island
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DOI：
10.1007/s13213-014-0829-8
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发表时间：
2015-03
影响因子：
3
通讯作者：
G. Bacci;Ettore Pagoto;M. Passaponti;P. Vannocci;A. Ugolini;A. Mengoni
中科院分区：
文献类型：
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作者：
G. Bacci;Ettore Pagoto;M. Passaponti;P. Vannocci;A. Ugolini;A. Mengoni
海洋海岸是高度动态的生态系统，桑迪是最多样化的生态系统之一。尽管桑迪作为海洋和陆地之间的过渡生态系统至关重要，但对海滩沉积物微生物组成的研究很少。为了提供第一个描述的微生物组成的滨海沉积物，我们调查了三个桑迪的细菌群落的组成，在Favignana岛，意大利，使用宏遗传学方法（终端限制性片段长度多态性，测序的16 S rRNA基因的Illumina-Solexa技术，功能基因检测，和定量实时PCR）。结果表明，调查海域细菌多样性丰富，主要由α-变形菌、γ-变形菌、黄杆菌和放线菌组成。宏成分析表明，配置文件的减少β多样性和增加的丰富性，以及分化的社区，沿着海到陆轴。特别是，Firmicutes和Proteobacteria的成员显示相对丰富的对比配置文件（减少和增加，分别）沿着海-陆轴的海滩。最后，对与氮和碳生物化学循环相关的基因（nifH、nosZ、pmoA/amoA）的搜索仅检测到氨单加氧酶序列（amoA）的存在，表明在一定程度上存在细菌氨氧化，可能是由于Nitrospira的成员，但缺乏固氮和反硝化作用。
Marine coasts represent highly dynamic ecosystems, with sandy beaches being one of the most heterogeneous. Despite the key importance of sandy beaches as transition ecosystems between sea and land, very few studies on the microbiological composition of beach sediments have been performed. To provide a first description of microbial composition of supralittoral sediments, we investigated the composition of bacterial communities of three sandy beaches, at Favignana Island, Italy, using metagenetic approaches (Terminal-Restriction Fragment Length Polymorphism, sequencing of 16S rRNA genes by Illumina-Solexa technology, functional genes detection, and quantitative Real-Time PCR). Results showed that the investigated beaches are harboring a rich bacterial diversity, mainly composed by members of classesAlphaproteobacteria,Gammaproteobacteria,FlavobacteriaandActinobacteria. The metagenetic analysis showed profiles of decreasing beta diversity and increasing richness, as well as a differentiation of communities, along the sea-to-land axis. In particular, members ofFirmicutesandProteobacteriadisplayed contrasting profiles of relative abundance (to decrease and to increase, respectively) along the sea-to-land axis of the beach. Finally, a search for the presence of genes related to the nitrogen and carbon biogeochemical cycle (nifH,nosZ,pmoA/amoA) detected the presence of ammonia monoxygenase sequences (amoA) only, suggesting the presence of bacterial ammonia oxidation to some extent, probably due to members ofNitrospira, but with the lack of nitrogen fixation and denitrification.