Synergistic action of cholera toxin B subunit (and Escherichia coli heat-labile toxin B subunit) and a trace amount of cholera whole toxin as an adjuvant for nasal influenza vaccine.
Synergistic action of cholera toxin B subunit (and Escherichia coli heat-labile toxin B subunit) and a trace amount of cholera whole toxin as an adjuvant for nasal influenza vaccine.
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霍乱毒素B亚基（及大肠杆菌不耐热毒素B亚基）与微量霍乱全毒素作为鼻流感疫苗佐剂的协同作用。
DOI：
10.1016/0264-410x(94)90118-x
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发表时间：
1994
期刊：
影响因子：
5.5
通讯作者：
T. Kurata
中科院分区：
文献类型：
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作者：
S. Tamura;Aya Yamanaka;Miyuki Shimohara;T. Tomita;K. Komase;Yusuke Tsuda;Yujiro Suzuki;Takashi Nagamine;K. Kawahara;H. Danbara;C. Aizawa;A. Oya;T. Kurata
研究了霍乱毒素B亚基（CTB）和大肠杆菌不稳定毒素（LTB） （2 μg）分别添加微量霍乱毒素（CT） （0.02 ~ 20 ng）对Balb/c小鼠流感灭活血凝素（HA）疫苗抗体（Ab）应答的佐剂作用。每只小鼠首次鼻内接种疫苗（1.5 μg）和含ct的CTB，并在4周内第二次单独接种疫苗。单独接种CTB疫苗一次接种不诱导抗ha IgA或IgG抗体反应，也不诱导血清中血凝抑制抗体反应。CT小于2 ng的疫苗也不能诱导Ab反应。另一方面，含有CT的CTB疫苗诱导了高Ab反应，其随CT剂量的增加而增加。此外，第二种疫苗诱导的应答比第一种疫苗高10倍以上，并且应答随CT剂量的增加而增加。在鼻洗液中发现了类似的增强局部抗ha IgA Ab反应。在LTB和CT之间也观察到这种协同效应。协同作用产生的Ab量被认为足以防止病毒感染。这些结果表明，含有微量CT（约0.1%）的CTB（或LTB）实际上可以用作人类抗流感鼻腔疫苗的有效佐剂。
Cholera toxin B subunit (CTB) andEscherichia coliheat-labile toxin (LTB) (2 μg), each supplemented with a trace amount of cholera toxin (CT) (0.02–20 ng), were examined for the adjuvant effect on antibody (Ab) response against influenza inactivated HA (haemagglutinin) vaccine in Balb/c mice. Each mouse received a primary intranasal (i.n.) inoculation of the vaccine (1.5 μg) and the CT-containing CTB and in 4 weeks a second i.n. inoculation of the vaccine alone. The primary inoculation of the vaccine with CTB alone did not induce either anti-HA IgA or IgG Ab response, or haemagglutination-inhibition Ab responses in the serum. The vaccine with less than 2 ng of CT also failed to induce Ab response. On the other hand, the vaccine with CT-containing CTB induced a high Ab response, which increased depending on the CT dose. Moreover, the second vaccine induced a response more than ten times higher than the primary one and the response increased depending on the CT dose. Similar enhancement was found in the local anti-HA IgA Ab response in the nasal wash. Such synergistic effects were observed also between LTB and CT. The amount of Ab produced by the synergism was considered to be enough to protect against virus infection. These results suggest that CTB (or LTB) containing a trace amount of CT (about 0.1%) can be used practically as a potent adjuvant for nasal vaccination of humans against influenza.
霍乱毒素 B 亚基对小鼠淋巴细胞增殖的抑制作用。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Woogen,SD;Ealding,W;Elson,CO
通讯作者：
Elson,CO