Non-specific adsorption on antibody surfaces for immunosensing
Non-specific adsorption on antibody surfaces for immunosensing
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DOI：
10.1016/0928-4931(95)00082-8
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发表时间：
1995-12-01
期刊：
影响因子：
--
通讯作者：
DeRossi, D
中科院分区：
文献类型：
--
作者：
Ahluwalia, A;Giusto, G;DeRossi, D
在涉及生物受体或识别分子的几乎所有情况下都会出现的非特异性吸附现象，参考免疫传感器的抗体表面进行了探讨。描述了两种不同类型的分析，三明治酶免疫测定和一系列直接全内反射荧光测量，以评估抗体表面上的非特异性吸附。在实验中，抗体包被的底物暴露于含有干扰剂的溶液中，并估计对结合位点表面密度的影响。
The phenomenon of non specific adsorption, which crops up in almost every case in which biological receptor or recognition molecules are involved, is probed with reference to antibody surfaces for immunosensors. Two different types of analysis, a sandwich enzyme immunoassay and a series of direct total internal reflection fluorescence measurements, to evaluate non-specific adsorption on antibody surfaces are described. In the experiments, antibody coated substrates are exposed to solutions containing interfering agents, and the effects on the surface density of binding sites are estimated.