Legionella tunisiensis sp nov and Legionella massiliensis sp nov., isolated from environmental water samples
Legionella tunisiensis sp nov and Legionella massiliensis sp nov., isolated from environmental water samples
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DOI：
10.1099/ijs.0.037853-0
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发表时间：
2012-12-01
影响因子：
2.8
通讯作者：
La Scola, Bernard
中科院分区：
文献类型：
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作者：
Campocasso, Angelique;Boughalmi, Mondher;La Scola, Bernard
根据显微镜外观、染色特征、不同温度下的无菌生长和mip、rpoB、16 S rRNA和rnpb基因序列以及23 S-5S区域，对两种阿米巴内生长细菌分离株LegA（T）（= DSM 24804（T）= CSUR P146（T））和LegM（T）（=DSM 24805（T）=CSUR P145（T））进行了表征。系统发育分析表明，这两个菌株位于军团菌科的半径。此外，对这些基因的分析产生了一致的数据，表明尽管菌株LegM（T）与菲氏军团菌ATCC 35072（T）特异性聚簇，而LegA（T）与鹦鹉螺军团菌ATCC 49596（T）聚簇，但在这些物种之间观察到的差异大于在该家族其他成员之间观察到的差异。综上所述，这些结果支持这两个分离株代表军团菌属新成员的提议，我们建议将其命名为LegM（T）的突尼斯军团菌新种（=DSM 24805（T）=CSUR P145（T））和LegA（T）的利氏军团菌新种（=DSM 24804（T）=CSUR P146（T））。
Two isolates of intra-amoeba-growing bacteria, LegA(T) (=DSM 24804(T)=CSUR P146(T)) and LegM(T) (=DSM 24805(T)=CSUR P145(T)), were characterized on the basis of microscopic appearance, staining characteristics, axenic growth at different temperatures and the sequences of the mip, rpoB, 16S rRNA and rnpb genes, as well as the 23S-5S region. Phylogenetic analysis showed that these two isolates lay within the radius of the family Legionellaceae. Furthermore, the analysis of these genes yielded congruent data that indicated that, although strain LegM(T) clusters specifically with Legionella feeleii ATCC 35072(T) and LegA(T) clusters with Legionella nautarum ATCC 49596(T), the divergence observed between these species was greater than that observed between other members of the family. Taken together, these results support the proposal that these two isolates represent novel members of the genus Legionella, and we propose to name them Legionella tunisiensis sp. nov. for LegM(T) (=DSM 24805(T)=CSUR P145(T)) and Legionella massiliensis sp. nov. for LegA(T) (=DSM 24804(T)=CSUR P146(T)).