Effect of vitamin D deficiency on in vitro labeling of chick intestinal proteins: analysis by two-dimensional electrophoresis.
Effect of vitamin D deficiency on in vitro labeling of chick intestinal proteins: analysis by two-dimensional electrophoresis.
复制标题
维生素 D 缺乏对鸡肠道蛋白体外标记的影响：二维电泳分析。
DOI：
10.1021/bi00521a030
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
DeLuca,HF
中科院分区：
文献类型：
--
作者：
Kendrick,NC;Barr,CR;Moriarity,D;DeLuca,HF
南希·C查尔斯·肯德里克巴尔，1* 多琳·莫里亚蒂，§和赫克托F. DeLuca* 摘要：维生素D3充满小鸡的十二指肠组织在体外标记2.5小时，3 H-标记的氨基酸，然后与组织严重维生素D缺乏小鸡孵育在相同的方式与14 C-标记的氨基酸。用双向电泳法分离酸性蛋白质，用McConkey双标记放射自显影法测定蛋白质中~ 3 H/~（14）C比值的变化。然后通过直接测量放射性来确定具有高同位素掺入的蛋白质的3 H/14 C比率。在沉淀级分中分离的100种蛋白质中，发现2种蛋白质的3 H/14 C比大于基线。蛋白1
Nancy C. Kendrick, Charles R. Barr, 1* Doreen Moriarity, § and Hector F. DeLuca* abstract: Duodenal tissue from vitamin D3 replete chicks was labeled in vitro for 2.5 h with 3H-labeled amino acids and then combined with tissue from severely vitamin D deficient chicks incubated in an identical fashion with 14C-labeled amino acids. Four double-labeled samples thus obtained were sep-arated into pellet and cytosol fractions by centrifugation, the acidic proteins ineach fraction separated by two-dimensional electrophoresis, and the separated proteins analyzed for changes in 3H/14C ratio by the McConkey method of double-label autoradiography. The 3H/14C ratios for proteins with high isotope incorporation were then determined by direct measurement of radioactivity. Of the 100 proteins resolved in the pellet fraction, two were found with 3H/14C ratios greater than base line. Protein 1