Cholinergic responsiveness of goblet cells during intestinal maturation.
Cholinergic responsiveness of goblet cells during intestinal maturation.
复制标题
肠道成熟过程中杯状细胞的胆碱能反应。
DOI：
10.1159/000242917
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Neutra,MR
中科院分区：
文献类型：
--
作者：
Phillips,TE;Phillips,TL;Neutra,MR
在成年大鼠肠道，胆碱能刺激加速分泌粘液的隐窝，但不是绒毛，杯状细胞。尚不清楚胎鼠或乳鼠的杯状细胞是否对胆碱能敏感。对幼鼠（妊娠20天至出生后30天）皮下注射卡巴胆碱，5分钟后固定肠道。通过半薄塑料切片的光学显微镜检查评估粘液分泌加速。回肠和结肠隐窝中的杯状细胞直到出生后20-25天才显示出成人样反应。回肠和结肠粘膜外植体在体外表现出与体内观察到的相同的年龄依赖性反应。胆碱能敏感性的发作发生在隐窝形成后，并始终限于隐窝杯状细胞。
In adult rat intestine, cholinergic stimulation accelerates discharge of mucus from crypt, but not villus, goblet cells. It was not known whether goblet cells in fetal or suckling rats are cholinergically sensitive. Rat pups (20 days of gestation to 30 postnatal days) were given subcutaneous injections of carbachol and the intestines were fixed 5 min later. Accelerated mucus secretion was assessed by light microscopy of semithin plastic sections. Goblet cells in ileal and colonic crypts did not show an adult-like response until 20–25 days after birth. Ileal and colonic mucosal explants in vitro showed age-dependent responses identical to those observed in vivo. The onset of cholinergic sensitivity occurred well after the formation of crypts and was always limited to crypt goblet cells.