Super-resolution microscopy of the synaptic active zone
Super-resolution microscopy of the synaptic active zone
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DOI：
10.3389/fncel.2015.00007
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发表时间：
2015-01-30
影响因子：
5.3
通讯作者：
Kittel, Robert J.
中科院分区：
文献类型：
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作者：
Ehmann, Nadine;Sauer, Markus;Kittel, Robert J.
大脑的功能依赖于化学突触上准确的信息传递。在突触前活性区（AZ），多种特化蛋白被组装成复杂的结构，这为突触传递的速度、精度和可塑性奠定了基础。钙通道对于激发-分泌耦合的启动至关重要，相应地，钙通道占据了AZ的中心位置。将定量功能研究与建模方法相结合，可以预测纳米尺度上的通道性质、数量甚至位置。然而，阐明周围蛋白质网络的纳米级组织需要直接的超微结构访问。没有这些信息，对分子突触结构-功能关系的认识仍然是不完整的。近年来，超分辨率显微镜（SRM）技术开始进入神经科学领域。这些方法结合了高空间分辨率和荧光显微镜的分子特异性。在这里，我们讨论如何使用SRM来获得关于AZ蛋白组织的信息
Brain function relies on accurate information transfer at chemical synapses. At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Calcium channels are pivotal for the initiation of excitation-secretion coupling and, correspondingly, capture a central position at the AZ. Combining quantitative functional studies with modeling approaches has provided predictions of channel properties, numbers and even positions on the nanometer scale. However, elucidating the nanoscopic organization of the surrounding protein network requires direct ultrastructural access. Without this information, knowledge of molecular synaptic structure-function relationships remains incomplete. Recently, super-resolution microscopy (SRM) techniques have begun to enter the neurosciences. These approaches combine high spatial resolution with the molecular specificity of fluorescence microscopy. Here, we discuss how SRM can be used to obtain information on the organization of AZ proteins