A Haspin promoter element induces tissue-specific methylation of a transcription region and the regulation of gene expression in mouse ova.
A Haspin promoter element induces tissue-specific methylation of a transcription region and the regulation of gene expression in mouse ova.
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DOI：
10.22074/cellj.2022.8444
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发表时间：
2022-09-12
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2
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HASPIN是最初在小鼠睾丸中鉴定的核丝氨酸-苏氨酸激酶。其193 bp DNA启动子元件（以下简称193 PE）调节雄性小鼠生殖细胞中的双向、同步基因表达。最近的研究表明，Haspin在体细胞中也有微量表达; HASPIN在卵母细胞中也有功能。Haspin表达受Haspin基因组DNA区域（包括体细胞）的组织特异性甲基化调节。本研究通过检测193 PE和报告基因转基因小鼠的甲基化状态，探讨193 PE与DNA甲基化的关系。在携带报告基因的体细胞（肝）中，193 PE诱导甲基化以及报告基因的微量表达。在睾丸中，193 PE诱导低甲基化和强烈的报告基因表达。使用人绒毛膜促性腺激素诱导雌性转基因小鼠排卵，评估卵子中HASPIN的表达。结果表明，193 PE诱导组织特异性甲基化，这导致报告基因在小鼠卵中表达。
HASPIN is a nuclear serine-threonine kinase originally identified in the mouse testis. Its 193 bp DNA promoter element (hereafter, 193PE) regulates bidirectional, synchronous gene expression in the germ cells of male mice. Recent studies have shown that Haspin is also expressed in trace amounts in somatic cells; HASPIN also functions in oocytes. Haspin expression is regulated by the tissue-specific methylation of Haspin genomic DNA regions, including somatic cells. This study investigated relationship between 193PE and DNA methylation by examining methylation status of transgenic mice carrying 193PE and a reporter gene. In somatic (liver) cells carrying the reporter gene, 193PE induced methylation as well as trace expression of the reporter gene. In the testis, 193PE induced hypomethylation and intense reporter gene expression. Expression of HASPIN in an egg was assessed using human chorionic gonadotrophin to induce ovulation in female transgenic mice. The results showed that 193PE induced tissue-specific methylation, which resulted in reporter gene expression in a mouse egg.