Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)
Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)
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小菜蛾 P-糖蛋白的分子克隆和表征（鳞翅目：小菜蛾科）
DOI：
10.3390/ijms141122891
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发表时间：
2013-11-01
影响因子：
5.6
通讯作者：
Wu, Qingjun
中科院分区：
文献类型：
--
作者：
Tian, Lixia;Yang, Jiaqiang;Wu, Qingjun
大环内酯如阿维菌素和伊维菌素构成一类重要的广谱杀虫剂。小菜蛾对阿维菌素、伊维菌素等合成杀虫剂的抗药性已成为小菜蛾防治的严重威胁。（鳞翅目：菜蛾科），十字花科植物的主要害虫。P-糖蛋白（P-glycoprotein，Pgp）是ABC转运蛋白超家族的一员，在两亲性外源性药物的清除中起着至关重要的作用，提示了靶生物的耐药机制。本研究克隆了小菜蛾Pgp基因PxPgp 1，并对其进行了结构分析。PxPgp 1的开放阅读框（ORF）由3774个核苷酸组成，编码一个1257个氨基酸的多肽。推导的PxPgp 1蛋白具有典型Pgp的结构特征，并在昆虫ABCB 1内聚集。PxPgp 1在所有发育阶段都有表达，在成年雄性中表达水平最高。PxPgp 1在中肠、马氏管和睾丸中高表达。阿维菌素处理后，小菜蛾菌株中PxPgp 1的表达量明显增加。此外，PxPgp 1的组成型表达显着较高的实验室选择和现场收集的抗性菌株相比，他们的敏感对应。
Macrocyclic lactones such as abamectin and ivermectin constitute an important class of broad-spectrum insecticides. Widespread resistance to synthetic insecticides, including abamectin and ivermectin, poses a serious threat to the management of diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), a major pest of cruciferous plants worldwide. P-glycoprotein (Pgp), a member of the ABC transporter superfamily, plays a crucial role in the removal of amphiphilic xenobiotics, suggesting a mechanism for drug resistance in target organisms. In this study, PxPgp1, a putative Pgp gene from P. xylostella, was cloned and characterized. The open reading frame (ORF) of PxPgp1 consists of 3774 nucleotides, which encodes a 1257-amino acid peptide. The deduced PxPgp1 protein possesses structural characteristics of a typical Pgp, and clusters within the insect ABCB1. PxPgp1 was expressed throughout all developmental stages, and showed the highest expression level in adult males. PxPgp1 was highly expressed in midgut, malpighian tubules and testes. Elevated expression of PxPgp1 was observed in P. xylostella strains after they were exposed to the abamectin treatment. In addition, the constitutive expressions of PxPgp1 were significantly higher in laboratory-selected and field-collected resistant strains in comparison to their susceptible counterpart.