Bifidobacterium affected the correlation between gut microbial composition, SCFA metabolism, and immunity in mice with DNFB-induced atopic dermatitis
Bifidobacterium affected the correlation between gut microbial composition, SCFA metabolism, and immunity in mice with DNFB-induced atopic dermatitis
复制标题
双歧杆菌影响 DNFB 诱导的特应性皮炎小鼠肠道微生物组成、SCFA 代谢和免疫力之间的相关性
DOI：
10.1016/j.fbio.2020.100648
复制
发表时间：
2021-06-01
期刊：
影响因子：
5.2
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Fang, Zhifeng;Li, Lingzhi;Chen, Wei
特应性皮炎（AD）的患病率增加，并已报道了使用益生菌干预的治疗策略。研究了不同双歧杆菌（B. breve，B. bifidum，B. animalis，B.婴儿和B.在2，4-二硝基氟苯（DNFB）诱导的AD（一种辅助性T细胞2型显性过敏性疾病）中探索了维生素B1）对肠道微生物变化的影响。口服双歧杆菌抑制皮肤增厚和肥大细胞浸润，阻断促炎细胞因子（IL-4、CCL 11、IL-13和CCL 22），增加IL-10和IFN-γ，并调节肠道中的微生物组和生态。基于16 S rRNA结果，DNFB处理导致肠道微生物生态失调，其特征在于厚壁菌门的比例减少和拟杆菌门的增加。还观察到与AD相关的核心微生物组（Dorea、Prevotella、Sutterella、Odoribacter和Pseudomonas）。B。breve和B.两歧菌处理增加了阿得勒克罗伊特氏菌、链球菌、乳杆菌、厌氧柄菌和厌氧干菌的相对丰度。参与果糖和甘露糖代谢、丙酸代谢和脂肪酸生物合成的功能模块被上调，并且这些可能与免疫调节和短链脂肪酸（SCFA）产生有关。综上所述，这些结果表明双歧杆菌处理影响肠道微生物组成和结构，改变肠道微生物生态系统，并且这些改变与炎症和SCFA产生密切相关。
The prevalence of atopic dermatitis (AD) has increased and a therapeutic strategy using probiotic intervention has been reported. The effects of different Bifidobacterium ( B. breve, B. bifidum, B. animalis, B. infants, and B. adolescentis ) on gut microbial changes were explored in 2,4-dinitrofluorobenzene (DNFB)-induced AD, a T helper type 2 -dominant allergic disease. Oral administration of Bifidobacterium suppressed skin thickening and mast cell infiltration, blocked pro -inflammatory cytokines (IL -4, CCL11, IL -13, and CCL22), increased IL -10 and IFN- gamma, and modulated the microbiome and ecology in the gut. Based on 16S rRNA results, DNFB treatment resulted in gut microbial dysbiosis, characterized by a decrease in the proportion of Firmicutes and an increase in Bacteroidetes . The core microbiome related to AD were also observed ( Dorea , Prevotella , Sutterella , Odoribacter , and Pseudomonas ) . B. breve and B. bifidum treatments increased the relative abundance of Adlercreutzia , Streptococcus , Lactobacillus , Anaerostipes , and Anaerotruncus . The functional modules involved in fructose and mannose metabolism, propanoate metabolism, and fatty acid biosynthesis were upregulated, and these might be related to immune regulation and short -chain fatty acids (SCFA) production. Taken together, these results suggested that Bifidobacterium treatments affected gut microbial composition and structure, altered gut microbial ecosystem, and these alterations were closely associated with inflammation and SCFA production.