CYTOKINE GENE-EXPRESSION IN EPIDERMIS WITH BIOLOGICAL EFFECTS FOLLOWING INJECTION OF NAKED DNA
CYTOKINE GENE-EXPRESSION IN EPIDERMIS WITH BIOLOGICAL EFFECTS FOLLOWING INJECTION OF NAKED DNA
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DOI：
10.1038/ng0695-161
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发表时间：
1995-06-01
期刊：
影响因子：
30.8
通讯作者：
VOGEL, JC
中科院分区：
文献类型：
--
作者：
HENGGE, UR;CHAN, EF;VOGEL, JC
表皮易于进行遗传操作并易于监测。由于猪皮在形态学上与人类皮肤非常相似，我们开发了一种在表皮中瞬时表达生物活性因子的方法。将裸质粒DNA直接注射到皮肤中后，DNA被表皮角质形成细胞摄取并瞬时高水平表达。将白细胞介素-8质粒DNA注射到皮肤中导致中性粒细胞募集的适当生物学应答，证明了功能效用。除了该模型的治疗用途之外，还可以在体内研究结构基因产物对表皮的生物学作用。
The epidermis is readily accessible for genetic manipulation and is easily monitored. Using pig skin because it is very similar to human skin morphologically, we have developed a method to transiently express biologically active factors in epidermis. Following direct injection of naked plasmid DNA into skin, DNA is taken up and transiently expressed at high levels by epidermal keratinocytes. Injection of interleukin-8 plasmid DNA into skin results in the appropriate biological response of neutrophil recruitment, demonstrating functional utility. In addition to this model's therapeutic uses, the biological effects of structural gene products on the epidermis could also be studied in vivo.