Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand
Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand
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DOI：
10.1111/j.1574-695x.2007.00375.x
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发表时间：
2008-04-01
影响因子：
--
通讯作者：
Day, Nicholas P. J.
中科院分区：
文献类型：
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作者：
Blacksell, Stuart D.;Luksameetanasan, Rungnapa;Day, Nicholas P. J.
恙虫病东方体是恙虫病的病原体，恙虫病是东南亚农村地区发热性疾病的主要病因。2003年至2005年期间，在泰国东北部（乌敦他尼省）和西部（德省）从恙虫病患者身上分离出23株恙虫病体。对分离株进行56- kda型特异性抗原基因ORF全序列测序，并进行系统发育分析。大多数分离株（15/23）为karp型，6株为gilliam型，TA716型和ta763型各1株。总体而言，序列上存在相当大的多样性，可与来自其他地区的丛林斑疹伤寒流行地区的菌株相媲美。两省间菌株分布差异无统计学意义（P=0.08, Fisher’s精确），菌株分布随分离年份的变化无统计学意义（P=0.80, Fisher’s精确）。在这种多样性中，也有从同一地理区域的患者培养的具有相同56-kDa基因型的分离株的例子。
Orientia tsutsugamushi is the causative agent of scrub typhus, a major cause of febrile illness in the rural areas of Southeast Asia. Twenty-three strains of O. tsutsugamushi were isolated from patients with scrub typhus in north-east (Udorn Thani province) and western Thailand (Tak province) between 2003 and 2005. The isolates were characterized by sequencing the entire ORF of the 56-kDa-type-specific antigen gene, followed by phylogenetic analysis. The majority (15/23) of isolates clustered with the Karp-type strain, six with a Gilliam-type strain and one each with the TA716- and TA763-type strains. Overall, there was considerable diversity in sequence, comparable to that seen in strains from across the rest of the scrub typhus-endemic world. There was no significant difference in the distributions of strains between the two provinces (P=0.08, Fisher's exact) nor a temporal change in distribution with year of isolation (P=0.80, Fisher's exact). Within this diversity there were also examples of isolates with identical 56-kDa genotypes that were cultured from patients from the same geographical areas.