Cross-Species Amplification of Microsatellite Loci for the Endangered Conifer, Taxus chinensis var. mairei (Taxaceae)
Cross-Species Amplification of Microsatellite Loci for the Endangered Conifer, Taxus chinensis var. mairei (Taxaceae)
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DOI：
10.1515/sg-2012-0036
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发表时间：
2012-12
期刊：
影响因子：
1
通讯作者：
Y. Wen;K. Uchiyama;G. Xu;S. Ueno;Wei Han;W. Xie;Y. Tsumura
中科院分区：
文献类型：
--
作者：
Y. Wen;K. Uchiyama;G. Xu;S. Ueno;Wei Han;W. Xie;Y. Tsumura
摘要 红豆杉作为一种经济濒危树种，其微卫星标记的研究取得了一定的成果。 maairei 的数量非常有限。我们开发了一套微卫星标记，有利于今后对该稀有物种的遗传分析。通过跨物种扩增方法从同属物种开发多态性基因座，并重新设计新的引物以测试潜在的无效等位基因。 15个位点显示多态性。在 48 名个体中测试的每个位点的等位基因数量从 2 到 23 个不等。观察到的杂合度 (Ho) 和预期杂合度 (He) 值的范围分别为 0.000 至 0.854 和 0.082 至 0.827。新设计的引物证实大多数可疑位点不存在无效等位基因。这些微卫星标记将有助于未来对该濒危物种的遗传分析和保护。
Abstract Being an economical and endangered species, microsatellite markers of Taxus chinensis var. mairei were very limited. We have developed a set of microsatellite markers, which was benefit for future genetic analysis of this rare species. Polymorphic loci were developed from congeneric species by cross-species amplification methods, and new primers were redesigned to test for potential null alleles. 15 loci showed polymorphism. The number of alleles per locus varied from 2 to 23 tested in 48 individuals. The observed heterozygosity (Ho) and expected heterozygosity (He) values ranged form 0.000 to 0.854 and 0.082 to 0.827, respectively. Newly redesigned primer confirmed that no null allele existed in most suspected loci. These microsatellite markers will be useful for future genetic analysis and conservation of this endangered species.