Phenethyl isothiocyanate protects against H2O2-induced insulin resistance in 3T3-L1 adipocytes
Phenethyl isothiocyanate protects against H2O2-induced insulin resistance in 3T3-L1 adipocytes
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异硫氰酸苯乙酯可防止 3T3-L1 脂肪细胞中 H2O2 诱导的胰岛素抵抗
DOI：
10.1080/09168451.2017.1372181
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Nagasawa Takashi
中科院分区：
文献类型：
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作者：
Nagami Moe;Ito Yoshiaki;Nagasawa Takashi
肥胖与全身性氧化应激有关，并导致胰岛素抵抗。异硫氰酸苯乙酯（PEITC）是一种天然的膳食异硫氰酸酯，通过激活核因子红细胞2相关因子2（Nrf 2）通路，提高细胞抗氧化应激的防御能力。然而，很少有证据表明抗氧化活性对葡萄糖代谢有有益的影响。在这里，我们测试了PEITC对3 T3-L1脂肪细胞中氧化应激引起的胰岛素诱导的葡萄糖摄取受损的预防潜力。PEITC处理增加了Nrf 2调节的抗氧化酶如γ-谷氨酰半胱氨酸合成酶、血红素加氧酶1、NAD（P）H：醌氧化还原酶1和谷胱甘肽S-转移酶的表达，并减少了H2 O2诱导的氧化应激。此外，PEITC恢复受损的胰岛素刺激的葡萄糖摄取，葡萄糖转运蛋白4的易位和胰岛素信号通过H2 O2。这些结果表明，PEITC通过在3 T3-L1脂肪细胞中的抗氧化活性保护由氧化应激损伤的胰岛素调节的葡萄糖代谢。
Obesity is associated with systemic oxidative stress and leads to insulin resistance. Phenethyl isothiocyanate (PEITC), a natural dietary isothiocyanate, has been shown to have beneficial effects in improving cellular defense activities against oxidative stress through activation of nuclear factor erythroid-2 related factor 2 (Nrf2) pathway. However, little evidence exists if the antioxidative activity has beneficial effects on glucose metabolism. Here, we tested the preventive potential of PEITC for impaired insulin-induced glucose uptake by oxidative stress in 3T3-L1 adipocytes. Treatment with PEITC increased the expression of antioxidative enzymes regulated by Nrf2 such as γ-glutamylcysteine-synthetase, heme oxygenase 1, NAD(P)H:quinone oxidoreductase 1 and glutathione S-transferase, and reduced oxidative stress induced by H2O2. Furthermore, PEITC restored impaired insulin-stimulated glucose uptake, translocation of glucose transporter 4 and insulin signaling by H2O2. These results indicate that PEITC protected insulin-regulated glucose metabolism impaired by oxidative stress through the antioxidative activity in 3T3-L1 adipocytes.