Sperm-egg fusion assay in mammals.
Sperm-egg fusion assay in mammals.
复制标题
哺乳动物精卵融合测定。
作者：
Naokazu Inoue;M. Okabe
作为构成人体的60万亿个细胞的代表，精子和卵子相遇、相互识别并融合创造新一代。因此，配子融合是一个极其重要的过程，必须毫无差错地发生才能启动生命活动。这可能会推动融合机制演变成一个持久而稳定的过程。同时，必须限制融合只发生在同一物种的两个配子之间。然而，受精中融合事件的分子基础尚未阐明。在本章中，我们描述了通过染色受精后肿胀的精子核来评估融合的方法。
As representatives of the 60 trillion cells that make a human body, a sperm and an egg meet, recognize each other, and fuse to create a new generation. Thus, gamete fusion is an extremely important process that must transpire without error to launch life activity. This may drive the fusion mechanism to evolve into an unfailing and steady process. At the same time, fusion must be restricted to occur only between two gametes of the same species. However, the molecular bases of the fusion event in fertilization have not yet been clarified. In this chapter, we describe the methods to evaluate fusion by staining the swollen sperm nuclei after fertilization.
影响因子：
2.7
作者：
Yamagata, K;Nakanishi, T;Okabe, M
通讯作者：
Okabe, M