ABSORPTION OF DIETARY QUERCETIN GLYCOSIDES AND QUERCETIN IN HEALTHY ILEOSTOMY VOLUNTEERS
ABSORPTION OF DIETARY QUERCETIN GLYCOSIDES AND QUERCETIN IN HEALTHY ILEOSTOMY VOLUNTEERS
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DOI：
10.1093/ajcn/62.6.1276
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发表时间：
1995-12-01
影响因子：
7.1
通讯作者：
KATAN, MB
中科院分区：
文献类型：
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作者：
HOLLMAN, PCH;DEVRIES, JHM;KATAN, MB
槲皮素是一种膳食抗氧化剂，可防止体外低密度脂蛋白氧化。荷兰老年男性摄入槲皮素与冠心病死亡率呈负相关。然而，槲皮素在人体中的吸收程度尚不清楚。本研究的目的是量化各种形式的槲皮素的吸收。研究了9名健康回肠造口术受试者，以避免结肠细菌造成的损失。他们遵循12天的无槲皮素饮食;在第4天，第8天和第12天，他们在早餐时接受了相当于89 mg糖苷配基的油炸洋葱（富含槲皮素糖苷），相当于100 mg糖苷配基的纯槲皮素芸香糖苷（茶中的主要槲皮素化合物），或100 mg纯槲皮素糖苷配基的补充，随机顺序。随后，参与者收集回肠造口术流出物和尿液13小时。在体外孵育槲皮素或其苷与胃肠液显示最小的降解。槲皮素的吸收，定义为口服摄入量减去回肠造口排泄量，并校正回肠造口袋内14%的降解，对于来自洋葱的槲皮素葡糖苷为52 +/- 15%，对于槲皮素芸香糖苷为17 +/- 15%，对于槲皮素苷元为24 +/- 9%。尿中槲皮素或其结合物的平均排泄量为吸收量的0.5%;尿中槲皮素的排泄量与回肠造口流出物中的排泄量呈负相关（r =-0.78，n = 27）。我们的结论是，人类吸收相当数量的槲皮素，并通过与葡萄糖结合增强吸收。
Quercetin is a dietary antioxidant that prevents oxidation of low-density lipoproteins in vitro. Intake of quercetin was inversely associated with coronary heart disease mortality in elderly Dutch men. However, the extent of absorption of quercetin in humans is unclear. The aim of this study was to quantify absorption of various forms of quercetin. Nine healthy ileostomy subjects were studied, to avoid losses caused by colonic bacteria. They followed a quercetin-free diet for 12 d; on days 4, 8, and 12 they received a supplement of fried onions at breakfast (rich in quercetin glucosides) equivalent to 89 mg aglycone, pure quercetin rutinoside (the major quercetin compound in tea) equivalent to 100 mg aglycone, or 100 mg pure quercetin aglycone, in random order. Subsequently, participants collected ileostomy effluent and urine for 13 h. In vitro incubations of quercetin or its glycosides with gastrointestinal fluids showed minimal degradation. Absorption of quercetin, defined as oral intake minus ileostomy excretion and corrected for 14% degradation within the ileostomy bag, was 52 +/- 15% for quercetin glucosides from onions, 17 +/- 15% for quercetin rutinoside, and 24 +/- 9% for quercetin aglycone. Mean excretion of quercetin or its conjugates in urine was 0.5% of the amount absorbed; quercetin excretion in urine was negatively correlated with excretion in ileostomy effluent (r = -0.78, n = 27). We conclude that humans absorb appreciable amounts of quercetin and that absorption is enhanced by conjugation with glucose.