Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms.
Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms.
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DOI：
10.1038/nmeth.2301
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发表时间：
2013-02
期刊：
影响因子：
48
通讯作者：
Meissner, Markus
中科院分区：
文献类型：
--
作者：
Andenmatten, Nicole;Egarter, Saskia;Jackson, Allison J;Jullien, Nicolas;Herman, Jean-Paul;Meissner, Markus
我们建立了一个条件性位点特异性重组系统的基础上二聚铬介导的重组顶端复形寄生虫弓形虫。使用一种新的单载体策略，允许配体依赖性，有效地去除感兴趣的基因，我们产生了三个敲除apicomplexan基因被认为是必不可少的宿主细胞入侵。我们的研究结果揭示了顶复门寄生虫中存在替代入侵途径。
We established a conditional site–specific recombination system based on dimerizable Cre–mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a novel single vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncover the existence of an alternative invasion pathway in apicomplexan parasites.