Detection of SARS-CoV-2 RNA Using RT-qPCR in Saliva Samples and Nasopharyngeal, Lingual, and Buccal Mucosal Swabs
Detection of SARS-CoV-2 RNA Using RT-qPCR in Saliva Samples and Nasopharyngeal, Lingual, and Buccal Mucosal Swabs
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DOI：
10.7883/yoken.jjid.2021.091
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发表时间：
2022-01-01
影响因子：
2.2
通讯作者：
Suzuki-Inoue, Katsue
中科院分区：
文献类型：
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作者：
Sasaki, Tomoyuki;Inoue, Osamu;Suzuki-Inoue, Katsue
2019年冠状病毒病的诊断是基于使用逆转录定量聚合酶链式反应在鼻咽拭子或唾液样本中检测到严重急性呼吸综合征冠状病毒2(SARS-CoV-2)RNA。鼻咽拭子应由医疗专业人员采集，并覆盖完整的个人防护装备(PPE)，而唾液样本可由患者自己采集，而无需个人防护装备。然而，对于不能遵循指示的人来说，收集唾液是困难的，包括婴儿或昏迷的患者。由于唾液的粘度很高，在实验室处理唾液样本时需要特别注意。为了解决这些问题，我们将舌头和口腔粘膜拭子(口腔拭子)与鼻咽拭子和唾液样本进行了比较。在13例鼻咽拭子SARS-CoV-2RNA阳性患者中，8例唾液和10例口腔拭子SARS-CoV-2RNA阳性，符合率为61.5%和76.9%。在8例唾液SARS-CoV-2 RNA阳性的患者中，7例(87.5%)在口腔拭子中检测到SARS-CoV-2。我们无法获得4名患者的唾液样本，但我们发现鼻咽拭子和口腔拭子的SARS-CoV-2阳性结果完全一致。因此，口腔拭子可用于SARS-CoV-2RNA的检测。
Coronavirus disease 2019 is diagnosed based on the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in nasopharyngeal swabs or saliva samples using reverse-transcription quantitative polymerase chain reaction. Nasopharyngeal swabs should be collected by medical professionals who are covered with full personal protective equipment (PPE), while saliva samples can be collected by patients themselves without any PPE. However, collecting saliva is difficult for people who are unable to follow instructions, including infants or unconscious patients. Owing to the high viscosity of saliva, special attention is required to handle saliva samples in laboratories. To solve these problems, we compared lingual and buccal mucosal swabs (oral swabs) with nasopharyngeal swabs and saliva samples. Among 13 patients who had a positive result for SARS-CoV-2 RNA in their nasopharyngeal swabs, 8 and 10 patients had a positive result for SARS-CoV-2 RNA in their saliva (concordance rate, 61.5%) and oral swabs (76.9%), respectively. Among the eight patients with a positive result for SARS-CoV-2 RNA in saliva, seven (87.5%) had SARS-CoV-2 detected in their oral swabs. We could not obtain saliva samples from four patients, but we found perfect concordance of SARS-CoV-2 positivity between the nasopharyngeal and oral swabs. Therefore, oral swabs can be used for SARS-CoV-2 RNA detection.