Jasmonate-dependent induction of indole glucosinolates in Arabidopsis by culture filtrates of the nonspecific pathogen Erwinia carotovora
Jasmonate-dependent induction of indole glucosinolates in Arabidopsis by culture filtrates of the nonspecific pathogen Erwinia carotovora
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DOI：
10.1104/pp.126.2.849
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发表时间：
2001-06-01
期刊：
影响因子：
7.4
通讯作者：
Palva, ET
中科院分区：
文献类型：
--
作者：
Brader, G;Tas, É;Palva, ET
来自植物病原菌Erwinia carotovora的激发子在拟南芥中协调色氨酸(Trp)生物合成途径的诱导，并尝试氧化基因。为了阐明这种病原体诱导的激活的生物学作用，我们表征了这种途径前体产生的次级防御代谢物，如Camalexin和吲哚硫代葡萄糖苷的产生。诱导子诱导后，3-吲哚甲基硫代葡萄糖苷(IGS)的含量明显增加，但仅有少量吲哚衍生的植物抗毒素Camalexin的积累。这种反应是由茉莉酸介导的，在茉莉酸不敏感的突变体COIL-1中缺乏IGS诱导。与此相一致，茉莉酸甲酯能够触发ICS在拟南芥中的积累。相比之下，乙烯和水杨酸似乎在反应中扮演着次要的角色。它们不会引起IGS水平的变化，茉莉酸甲酯或激发子诱导的IGS在NnhC和乙烯不敏感的ein2-1突变株中的积累与野生型相似。IGS和其他硫代葡萄糖苷的分解产物能够抑制胡萝卜素乳杆菌的生长。这些结果表明，IGS在抵御细菌病原体方面具有重要作用。
Elicitors from the plant pathogen Erwinia carotovora trigger coordinate induction of the tryptophan (Trp) biosynthesis pathway and Try oxidizing genes in Arabidopsis. To elucidate the biological role of such pathogen-induced activation we characterized the production of secondary defense metabolites such as camalexin and indole glucosinolates derived from precursors of this pathway. Elicitor induction was followed by a specific increase in 3-indolylmethylglucosinolate (IGS) content, but only a barely detectable accumulation of the indole-derived phytoalexin camalexin. The response is mediated by jasmonic acid as shown by lack of IGS induction in the jasmonate-insensitive mutant coil-1. In accordance with this, methyl jasmonate was able to trigger ICS accumulation in Arabidopsis. In contrast, ethylene and salicylic acid seem to play a minor role in the response. They did not trigger alterations in IGS levels, and methyl jasmonate- or elicitor-induced IGS accumulation in NnhC and ethylene-insensitive ein2-1 mutant plants was similar as in the wild type. The breakdown products of IGS and other glucosinolates were able to inhibit growth of E. carotovora. The results suggest that IGS is of importance in the defense against bacterial pathogens.