Increased sensitivity in proton transfer reaction mass spectrometry by using a novel focusing quadrupole ion funnel
Increased sensitivity in proton transfer reaction mass spectrometry by using a novel focusing quadrupole ion funnel
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使用新型聚焦四极离子漏斗提高质子转移反应质谱分析的灵敏度
DOI：
10.1021/acs.analchem.2c01893
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发表时间：
2022
影响因子：
7.4
通讯作者：
Yannan Chu
中科院分区：
文献类型：
--
作者：
Xun Bao;Qiangling Zhang;Qu Liang;Qin Sun;Wei Xu;Yan Lu;Lei Xia;Yawei Liu;Xue Zou;Chaoqun Huang;Chengyin Shen;Yannan Chu
提高质子转移反应质谱（PTR-MS）的灵敏度是一个重要的发展方向。我们开发了一种新的漂移管称为聚焦四极离子漏斗（FIGH-IF）用于PTR-MS，以提高灵敏度。中频由20层不锈钢电极组成，每层有4个四分之一环。前6层具有恒定的22 mm的内孔直径;后14层将内径逐渐减小至8 mm。通过改变电压加载方法，该中频漂移管还可以在直流（DC）模式（类似于传统的漂移管）和离子漏斗（IF）模式（类似于传统的离子漏斗漂移管）下工作。仿真结果表明，与其他两种模式相比，中频模式的传输效率得到了显著提高。进一步的实验表明，柠檬烯的子离子倾向于转换成较小的m/z碎片离子在较高的电压为DC和IF模式。然而，与DC和IF模式不同，对于FQ-IF，子离子的分布在较高电压下是稳定的。换句话说，用于RF-IF的较高RF电压将不会增加离子的碰撞能量。此外，对于12种测试化合物，与DC模式相比，对于12种测试化合物，对于12种测试化合物，与IF模式相比，对于12种测试化合物，灵敏度的改善范围为13.8至87.9倍，以及1.7至4.8倍。与直流模式相比，中频模式的检测限（LOD）提高了2.7至35.7倍。该方法为提高PTR-MS和其他质谱仪的灵敏度提供了有价值的参考。
Sensitivity enhancement in proton transfer reaction mass spectrometry (PTR-MS) is an important development direction. We developed a novel drift tube called a focusing quadrupole ion funnel (FQ-IF) for use in PTR-MS to improve the sensitivity. The FQ-IF consists of 20 layers of stainless steel electrodes, and each layer has 4 quarter rings. The first 6 layers have a constant inner hole diameter of 22 mm; the latter 14 layers taper the inner diameter down to 8 mm. The FQ-IF drift tube can also operate in the direct current (DC) mode (similar to a conventional drift tube) and ion funnel (IF) mode (similar to a conventional ion funnel drift tube) by changing the voltage loading method. The simulation results show that the transmission efficiency of the FQ-IF is significantly improved compared to that of the other two modes. Further experiments show that the product ions of limonene tend to convert into smallerm/zfragment ions at higher voltages for the DC and IF modes. However, unlike the DC and IF modes, the distribution of product ions is stable at higher voltages for the FQ-IF. In other words, a higher RF voltage for the FQ-IF will not increase the collision energy of ions. In addition, the improvements in sensitivity for the FQ-IF range from 13.8 to 87.9 times compared to the DC mode and from 1.7 to 4.8 times compared to the IF mode for the 12 test compounds. The improvements in the limit of detection (LOD) for the FQ-IF range from 2.7 to 35.7 times compared to the DC mode. The FQ-IF provides a valuable reference for improving the sensitivity of PTR-MS and other mass spectrometers.