Ultrahigh sensitivity made simple: nanoplasmonic label-free biosensing with an extremely low limit-of-detection for bacterial and cancer diagnostics
Ultrahigh sensitivity made simple: nanoplasmonic label-free biosensing with an extremely low limit-of-detection for bacterial and cancer diagnostics
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DOI：
10.1088/0957-4484/20/43/434015
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发表时间：
2009-10-28
期刊：
影响因子：
3.5
通讯作者：
Dmitriev, A.
中科院分区：
文献类型：
--
作者：
Chen, S.;Svedendahl, M.;Dmitriev, A.
我们提出了一个简单而强大的生物传感方案，其检测下限低至几pg cm（-2）（或几十阿托摩尔cm（-2）），基于光学无标记生物检测与局部表面等离子体共振。该方案利用具有成本效益的光学元件，包括一个白色光源，一个适当功能化的传感器表面封闭在一个简单的射流芯片，和光谱分析仪。传感器表面是由一个自下而上的纳米纤维技术与孔掩模胶体光刻。尽管其简单，该方法能够可靠地检测在低皮摩尔和飞摩尔浓度下的蛋白质-蛋白质结合事件，这通过在细菌金黄色葡萄球菌的外表面上发现的细胞外粘附蛋白（EAP）和前列腺特异性抗原（PSA）的无标记检测来举例说明，PSA被认为是前列腺癌标志物。这些实验为医疗点诊断应用的超灵敏而紧凑的生物检测平台铺平了道路。
We present a simple and robust scheme for biosensing with an ultralow limit-of-detection down to several pg cm(-2) (or several tens of attomoles cm(-2)) based on optical label-free biodetection with localized surface plasmon resonances. The scheme utilizes cost-effective optical components and comprises a white light source, a properly functionalized sensor surface enclosed in a simple fluidics chip, and a spectral analyzer. The sensor surface is produced by a bottom-up nanofabrication technique with hole mask colloidal lithography. Despite its simplicity, the method is able to reliably detect protein-protein binding events at low picomolar and femtomolar concentrations, which is exemplified by the label-free detection of the extracellular adherence protein (EAP) found on the outer surface of the bacterium Staphylococcus aureus and of prostate-specific antigen (PSA), which is believed to be a prostate cancer marker. These experiments pave the way towards an ultra-sensitive yet compact biodetection platform for point-of-care diagnostics applications.