DNA repair in reduced genome: The Mycoplasma model
DNA repair in reduced genome: The Mycoplasma model
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DOI：
10.1016/j.gene.2005.06.012
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发表时间：
2005-11-07
期刊：
影响因子：
3.5
通讯作者：
Agnez-Lima, LF
中科院分区：
文献类型：
--
作者：
Carvalho, FM;Fonseca, MM;Agnez-Lima, LF
基因组减少的细菌的出现，比如在支原体中发现的，提出了一个问题，即哪些基因应该足以保证维持生命所必需的基因组稳定性。这项工作的目的是比较九个支原体基因组的DNA修复基因。对6种支原体（基因组可在GenBank上找到）、滑膜支原体（M. synoviae）和2株All进行了计算机分析。其基因组最近分别由巴西国家基因组计划联盟和南方基因组调查计划（巴西）测序。考虑到这种简化的基因组模型，我们的比较分析表明，活体呼叫所需的DNA完整性主要通过核苷酸切除修复（NER）来维持，这是唯一完整的修复途径。此外，一些参与碱基切除修复（BER）和重组的酶也存在，可以补充NER的活性。仅在生殖支原体和肺炎支原体中观察到缺乏RecR和RecR样orf，这可能与这两个物种之间观察到的基因顺序的保守有关。我们还通过横向转移事件获得了最近在肺分枝杆菌和穿透分枝杆菌中获得ogt基因的系统发育证据。总的来说，修复基因的存在或不存在是所有被分析物种共有的，这表明大多数修复基因的缺失是一个祖先事件，发生在支原体物种分化之前。(c) 2005年Elsevier B.V.出版
The occurrence of bacteria with a reduced genome, such as that found in Mycoplasmas, raises the question as to which genes should be enough to guarantee the genomic stability indispensable for the maintenance of life. The aim of this work was to compare nine Mycoplasma genomes in regard to DNA repair genes. An in silico analysis was done using six Mycoplasma species, whose genomes are accessible at GenBank, and M. synoviae, and two strains of All. hyopneumoniae, whose genomes were recently sequenced by The Brazilian National Genome Project Consortium and Southern Genome Investigation Program (Brazil) respectively. Considering this reduced genome model, Our comparative analysis Suggests that the DNA integrity necessary for lire call be primarily maintained by nucleotide excision repair (NER), which is the only complete repair pathway. Furthemore, some enzymes involved with base excision repair (BER) and recombination are also present and can complement the NER activity. The absence of RecR and RecO-like ORFs was observed only in M. genitalium and M pneumoniae, which can be involved with the conservation of gene order observed between these two species. We also obtained phylogenetic evidence for the recent acquisition of the ogt gene in M. pulmonis and M. penetrans by a lateral transference event. In general, the presence or nonexistence of repair genes is shared by all species analyzed, Suggesting that the loss of the majority of repair genes was an ancestral event, which occurred before the divergence of the Mycoplasma species. (c) 2005 Published by Elsevier B.V.