Plasma microRNA profiling of pediatric patients with immune thrombocytopenic purpura
Plasma microRNA profiling of pediatric patients with immune thrombocytopenic purpura
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DOI：
10.1097/mbc.0000000000000069
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发表时间：
2014-06-01
影响因子：
1.1
通讯作者：
Aktekin, Elif
中科院分区：
文献类型：
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作者：
Bay, Ali;Coskun, Enes;Aktekin, Elif
免疫性血小板减少性紫癜（ITP）是儿童常见的获得性自身免疫性出血性疾病。microRNAs（miRNAs）是存在于细胞和循环中的小分子RNA，在蛋白质合成和调节中发挥作用。在这项研究中，我们的目的是确定儿童ITP的生物标志物，比较患有ITP的儿童与健康儿童的血浆miRNA水平。自2011年7月起，加济安泰普大学儿科血液肿瘤科随访的86例ITP患者和56例健康儿童共入组本研究。将86例ITP患者分为急性ITP（aITP）组43例和慢性ITP（cITP）组43例。通过RT-PCR（定量RT-PCR）技术研究379种miRNAs的血浆表达水平，并在aITP、cITP和对照组之间进行比较。对于所有miRNA，将分析芯片中分别三组的原始定量循环值的平均值作为参考基因值，并根据该值进行归一化。在所研究的7种miRNA（miR-302 c-3 p、miR-483- 5 p、miR-410、miR-544 a、miR-302 a-3 p、miR-223- 3 p和miR-597）中，在组间的表达水平方面检测到统计学显著性差异。在所有三组中，发现miR-302 c-3 p和miR-483- 5 p的表达率超过95%，miR-410的表达率超过75%，miR-544、miR-302 a-3 p和miR-223- 3 p的表达率超过40%。检测aITP和cITP患者与健康儿童血浆miRNA水平的显著差异可能为预测疾病病程、确定疾病病因和开发新的治疗方式提供有用的信息。
Immune thrombocytopenic purpura (ITP) is a commonly acquired autoimmune bleeding disorder in children. MicroRNAs (miRNAs) are small RNAs which are found in cells and circulation, and play a role in protein synthesis and regulation. In this study, we aimed to determine a biomarker for childhood ITP comparing the plasma miRNA levels of children having ITP with healthy children. A total of 86 patients with ITP and 56 healthy children followed up by the Department of Pediatric Hematology and Oncology in University of Gaziantep since July 2011 were enrolled in the study. The 86 patients with ITP were evaluated in two groups as 43 acute ITP (aITP) and 43 chronic ITP (cITP) patients. Plasma expression levels of 379 miRNAs were investigated by RT-PCR (quantitative RT-PCR) technique and they were compared between aITP, cITP, and control groups. For all miRNAs, the average of raw quantification cycle values of three groups separately in the analysis chip was accepted as the reference gene value, and normalization was done according to this value. Statistically significant differences were detected in seven miRNAs (miR-302c-3p, miR-483-5p, miR-410, miR-544a, miR-302a-3p, miR-223-3p, and miR-597) investigated between the groups with respect to the expression levels. The expression rates were found to be over 95% in miR-302c-3p and miR-483-5p, over 75% in miR-410, and over 40% in miR-544, miR-302a-3p, and miR-223-3p in all three groups. The detection of significant differences between plasma miRNA levels of aITP and cITP patients and healthy children may provide useful information in the prediction of the course of disease, determination of disease etiopathogenesis, and the development of new therapeutic modalities.