Determination of seventeen food additives in spice and drink by high performance liquid chromatography
Determination of seventeen food additives in spice and drink by high performance liquid chromatography
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发表时间：
2010
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通讯作者：
L. Ying
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作者：
L. Ying
目的：建立高效液相色谱同时测定调味品和饮料中17种食品添加剂的方法。方法：样品用水提取，采用Zorbax Eclipse C18柱，以甲醇-0.04 mol/L醋酸铵为流动相，梯度洗脱，流速1.0 ml/min，柱温35℃。结果：所有食品添加剂均呈良好的线性关系（r0.999）。检出限(LOD)在0.013 mg/kg～0.101 mg/kg(s/n=3)范围内。香料和饮料样品的平均回收率为90.1%～105%，相对标准偏差(RSD)小于5.1%。结论：该方法简单、快速、重复性好，可用于香料和饮料中食品添加剂的分析。
Objective:To develop a method for simultaneous determination of seventeen food additives in spice and drink by high performance liquid chromatography.Methods: Sample was extracted with water,and the analysis was performed on a Zorbax Eclipse C18 column using methanol-0.04 mol/L ammonium acetate as the mobile phase with a gradient elution with a flow rate of 1.0 ml/min and column temperature of 35℃.Results: All the food additives had a good linear relationship(r0.999).The detection limits(LOD) were in range of 0.013 mg/kg～0.101 mg/kg(s/n=3).The average recoveries for spice and drink samples ranged from 90.1%～105% with relative standard deviations(RSD) less than 5.1%.Conclusion: The method is simple,rapid,repeatable and which could be applied for the analysis of food additives in spice and drink.