Cellular Responses of Human Lymphatic Endothelial Cells to Carbon Nanomaterials
Cellular Responses of Human Lymphatic Endothelial Cells to Carbon Nanomaterials
复制标题
DOI：
10.3390/nano10071374
复制
发表时间：
2020-07
期刊：
影响因子：
5.3
通讯作者：
Mahoko Sano;Makoto Izumiya;H. Haniu;Katsuya Ueda;Kosuke Konishi;Haruka Ishida;C. Kuroda;Takeshi Uemu
中科院分区：
文献类型：
--
作者：
Mahoko Sano;Makoto Izumiya;H. Haniu;Katsuya Ueda;Kosuke Konishi;Haruka Ishida;C. Kuroda;Takeshi Uemu
在追求碳纳米材料（CNM）的医学应用时，需要克服的最大挑战之一是安全性。特别是，当考虑在药物递送系统（DDS）中使用CNM时，在蓄积部位的安全性评价是一个重要步骤。在这项研究中，我们评估了碳纳米角（CNH）的毒性，这是潜在的DDS，使用人淋巴结内皮细胞，已报告积累CNM，作为纤维状，多壁碳纳米管（MWCNT）和颗粒炭黑（CB）的比较。还使用两种类型的CNH（未处理的和氧化的）来评估不同表面特性的影响。在纤维状多壁碳纳米管中，观察到细胞生长抑制以及炎性细胞因子基因的表达，如在先前的报告中。相反，未观察到颗粒CB和CNHs的显著毒性，这与CB对血管内皮细胞的细胞毒性的报道不同。这些结果表明：（1）淋巴内皮细胞需要与其他内皮细胞分开检测，以进行纳米材料的安全性评价;（2）CNH作为DDS的潜力。
One of the greatest challenges to overcome in the pursuit of the medical application of carbon nanomaterials (CNMs) is safety. Particularly, when considering the use of CNMs in drug delivery systems (DDSs), evaluation of safety at the accumulation site is an essential step. In this study, we evaluated the toxicity of carbon nanohorns (CNHs), which are potential DDSs, using human lymph node endothelial cells that have been reported to accumulate CNMs, as a comparison to fibrous, multi-walled carbon nanotubes (MWCNTs) and particulate carbon black (CB). The effect of different surface characteristics was also evaluated using two types of CNHs (untreated and oxidized). In the fibrous MWCNT, cell growth suppression, as well as expression of inflammatory cytokine genes was observed, as in previous reports. In contrast, no significant toxicity was observed for particulate CB and CNHs, which was different from the report of CB cytotoxicity in vascular endothelial cells. These results show that (1) lymph endothelial cells need to be tested separately from other endothelial cells for safety evaluation of nanomaterials, and (2) the potential of CNHs as DDSs.