A closed conformation for the Pol λ catalytic cycle
A closed conformation for the Pol λ catalytic cycle
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DOI：
10.1038/nsmb876
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发表时间：
2005-01-01
影响因子：
16.8
通讯作者：
Pedersen, LC
中科院分区：
文献类型：
--
作者：
Garcia-Diaz, M;Bebenek, K;Pedersen, LC
Pollambda是X家族的一个成员，被认为在DNA修复过程中填补了短的缺口。在这里，我们报告晶体结构的Pollambda代表三个步骤，在填补一个单核苷酸的差距。这些结构表明，不同于其他DNA聚合酶，Pollambda在催化过程中不经历大的子域运动，它们提供了一个明确的表征的几何形状和立体化学的在线核苷酸转移反应。
Pollambda is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pollambda representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pollambda does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction.