Pyrosequencing Analysis of the Bacteria Communities in the Guts of Honey Bees Apis cerana and Apis mellifera in Korea
Pyrosequencing Analysis of the Bacteria Communities in the Guts of Honey Bees Apis cerana and Apis mellifera in Korea
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DOI：
10.1007/s12275-012-2188-0
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发表时间：
2012-10-01
影响因子：
3
通讯作者：
Weon, Hang-Yeon
中科院分区：
文献类型：
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作者：
Ahn, Jae-Hyung;Hong, In-Pyo;Weon, Hang-Yeon
采用焦磷酸测序法对亚洲蜜蜂（Apis cerana）和欧洲蜜蜂（Apis mellifera）成虫和幼虫肠道内的细菌群落进行了研究。大多数肠道细菌的16S rRNA基因序列与已知的蜜蜂特异性序列高度相似，并且与巴氏杆菌或乳酸菌（LAB）有关。幼虫肠道的操作分类单位（otu，定义相似度为97%）数量（6或9）低于成虫肠道（18或20），并且与巴氏杆菌相关的otu在幼虫肠道中频率较高，而与实验室相关的otu在成虫肠道中频率较高。蜜蜂肠道中乳球菌、巴尔通体、螺旋体、肠杆菌科和黄杆菌科相关otu的频率较高，而蜜蜂肠道中双歧杆菌和毛螺旋体相关otu的频率较高。成年蜜蜂中、后肠细菌群落结构在蜜蜂中、后肠间差异不显著，而在蜜蜂中差异显著。上述结果证实了之前的观察结果，即蜜蜂肠道由几种特定的细菌群主导，也表明OTUs的相对丰度会因发育阶段、肠道内位置和蜜蜂种类的不同而发生显著变化。讨论了利用肠道细菌群落作为蜜蜂健康指标的可能性。
The bacterial communities in the guts of the adults and larvae of the Asian honey bee Apis cerana and the European honey bee Apis mellifera were surveyed by pyrosequencing the 16S rRNA genes. Most of the gut bacterial 16S rRNA gene sequences were highly similar to the known honey bee-specific ones and affiliated with Pasteurellaceae or lactic acid bacteria (LAB). The numbers of operational taxonomic units (OTUs, defined at 97% similarity) were lower in the larval guts (6 or 9) than in the adult guts (18 or 20), and the frequencies of Pasteurellaceae-related OTUs were higher in the larval guts while those of LAB-related OTUs in the adult guts. The frequencies of Lactococcus, Bartonella, Spiroplasma, Enterobacteriaceae, and Flavobacteriaceae-related OTUs were much higher in A. cerana guts while Bifidobacterium and Lachnospiraceae-related OTUs were more abundant in A. mellfera guts. The bacterial community structures in the midguts and hindguts of the adult honey bees were not different for A. cerana, but significantly different for A. mellifera. The above results substantiated the previous observation that honey bee guts are dominated by several specific bacterial groups, and also showed that the relative abundances of OTUs could be markedly changed depending on the developmental stage, the location within the gut, and the honey bee species. The possibility of using the gut bacterial community as an indicator of honey bee health was discussed.