Metagenomic analysis reveals wastewater treatment plants as hotspots of antibiotic resistance genes and mobile genetic elements
Metagenomic analysis reveals wastewater treatment plants as hotspots of antibiotic resistance genes and mobile genetic elements
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DOI：
10.1016/j.watres.2017.07.002
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发表时间：
2017-10-15
期刊：
影响因子：
12.8
通讯作者：
Yuan, Zhiguo
中科院分区：
文献类型：
--
作者：
Guo, Jianhua;Li, Jie;Yuan, Zhiguo
抗生素的大量使用导致其持续释放到环境中，并随后广泛出现抗生素抗性细菌（ARB）、抗生素抗性基因（ARG）和移动的遗传元件（MGE）。本研究采用Illumina高通量测序技术，研究了全规模污水处理厂活性污泥和厌氧消化污泥中ARGs和MGE的广谱谱。开发了一个用于鉴定抗生素耐药决定簇的管道，该管道包括四个类别：基因转移潜力、ARG潜力、ARG途径和ARG系统发育起源。宏基因组分析表明，活性污泥和消化污泥中微生物群落结构不同，ARG和MGEs的种类和发生率也不同。在活性污泥中共鉴定出42种ARG亚型，而在消化污泥中检测到51种ARG亚型。此外，MGE包括质粒、转座子、整合子。（intil）和插入序列（例如ISSsp 4、ISMsa 21和ISMba 16）在两个污泥样品中是丰富的。网络分析结果表明，某些环境细菌（如梭菌属和亚硝化单胞菌属）可能是多种ARGs的潜在宿主。这些发现增加了我们对WWTP作为ARG和MGE热点的理解，并有助于防止其释放到下游环境中。(C)2017爱思唯尔有限公司版权所有
The intensive use of antibiotics results in their continuous release into the environment and the subsequent widespread occurrence of antibiotic resistant bacteria (ARB), antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs). This study used Illumina high-throughput sequencing to investigate the broad-spectrum profiles of both ARGs and MGEs in activated sludge and anaerobically digested sludge from a full-scale wastewater treatment plant. A pipeline for identifying antibiotic resistance determinants was developed that consisted of four categories: gene transfer potential, ARG potential, ARGs pathway and ARGs phylogenetic origin. The metagenomic analysis showed that the activated sludge and the digested sludge exhibited different microbial communities and changes in the types and occurrence of ARGs and MGEs. In total, 42 ARGs subtypes were identified in the activated sludge, while 51 ARG subtypes were detected in the digested sludge. Additionally, MGEs including plasmids, transposons, integrons. (intil) and insertion sequences (e.g. ISSsp4, ISMsa21 and ISMba16) were abundant in the two sludge samples. The co-occurrence pattern between ARGs and microbial taxa revealed by network analysis indicated that some environmental bacteria (e.g. Clostridium and Nitrosomonas) might be potential hosts of multiple ARGs. The findings increase our understanding of WWTPs as hotspots of ARGs and MGEs, and contribute towards preventing their release into the downstream environment. (C) 2017 Elsevier Ltd. All rights reserved.