A Novel Electrochemical Sensor Based on a Cerium Oxide/Gold/Carbon Nanocompositefor the Detection of Hydroxyl Free Radicals
A Novel Electrochemical Sensor Based on a Cerium Oxide/Gold/Carbon Nanocompositefor the Detection of Hydroxyl Free Radicals
复制标题
基于氧化铈/金/碳纳米复合材料的新型电化学传感器，用于检测羟基自由基
DOI：
10.1149/1945-7111/acca4c
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发表时间：
2023
影响因子：
3.9
通讯作者：
Kim, Dong-Shik
中科院分区：
文献类型：
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作者：
Ghaedamini, Hamidreza;Alba-Rubio, Ana C.;Kim, Dong-Shik
羟基自由基（· OH）是维持人体细胞正常活动的重要化学物质;然而，· OH的浓度过高会破坏细胞的正常功能，导致各种疾病，包括肝脏和心脏疾病，癌症和神经系统疾病。因此，作为生物标志物的· OH的检测对于这些严重疾病的早期诊断至关重要。在此，一种新型的电化学传感器，包括氧化铈纳米团簇，金纳米粒子，和高导电性碳的复合物被开发用于检测· OH。采用循环伏安法（CV）和电化学阻抗谱（EIS）表征了复合物与· OH自由基相互作用产生的信号。CV结果表明，该传感器能够准确、选择性地检测芬顿反应中的· OH。该传感器在0.05 - 0.5 mM和0.5 - 5 mM范围内电流峰与· OH浓度呈线性关系，检测限（LOD）为58 μM。此外，电化学阻抗谱研究表明，该电化学传感器可以区分· OH和类似的活性氧（ROS），如过氧化氢（H2 O2）。还值得一提的是，其他优点，如传感器的再现性，重复性和稳定性得到了证实。
Hydroxyl radicals (• OH) are well known as crucial chemicals for maintaining the normal activities of human cells; however, the excessive concentration of• OH disrupts their normal function, causing various diseases, including liver and heart diseases, cancers, and neurological disorders. The detection of• OH as a biomarker is thus essential for the early diagnosis of these serious conditions. Herein, a novel electrochemical sensor comprising a composite of cerium oxide nanoclusters, gold nanoparticles, and a highly conductive carbon was developed for detecting• OH. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed to characterize the signals generated by the interaction of the composite with• OH radicals. The CV results revealed that the developed sensor could accurately and selectively detect• OH in the Fenton reaction. The sensor demonstrated a linear relationship between the current peak and• OH concentration in the range 0.05− 0.5 mM and 0.5− 5 mM with a limit of detection (LOD) of 58 μM. In addition, EIS studies indicated that this electrochemical sensor could distinguish between• OH and similar reactive oxygen species (ROS), like hydrogen peroxide (H 2 O 2). It is also worth mentioning that additional merits, such as reproducibility, repeatability, and stability of the sensor were confirmed.