Nanolithography using protease etching of protein surfaces
Nanolithography using protease etching of protein surfaces
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DOI：
10.1021/nl034640m
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发表时间：
2003-12-01
期刊：
影响因子：
10.8
通讯作者：
Gheber, LA
中科院分区：
文献类型：
--
作者：
Ionescu, RE;Marks, RS;Gheber, LA
我们首次提出了使用蛋白水解酶在蛋白质表面产生纳米大小的凹陷。这是通过将胰酶通过扫描探针显微镜控制的纳米管输送到干燥的牛血清白蛋白膜上实现的。我们建议一个全面的模型，解释所涉及的过程，并提供控制这些过程的参数的方法。酶纳米光刻技术在纳米生物技术领域应该是有用的，例如纳米生物芯片、纳米流体和芯片实验室。
We present for the first time the use of a proteolytic enzyme to create nanometer-size depressions in a protein surface. This was achieved by delivering trypsin via a nanopipet, controlled with a scanning probe microscope, to a dried bovine serum albumin film. We suggest a comprehensive model, explaining the processes involved and providing ways to control the parameters governing them. Enzyme nanolithography should be useful in areas of nanobiotechnology, such as nanobiochips, nanofluidics, and lab-on-a-chip.