Significance of the intact polypeptide chains of human fibrinogen in ADP-induced platelet aggregation
Significance of the intact polypeptide chains of human fibrinogen in ADP-induced platelet aggregation
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人纤维蛋白原完整多肽链在 ADP 诱导血小板聚集中的意义
DOI：
10.1182/blood.v49.4.635.bloodjournal494635
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发表时间：
1977
期刊：
影响因子：
20.3
通讯作者：
B. Lipinski
中科院分区：
文献类型：
--
作者：
S. Niewiarowski;A. Budzynski;B. Lipinski
洗涤的人血小板悬浮液中存在人纤维蛋白原是ADP诱导的血小板聚集的必要条件。用纤溶酶消化纤维蛋白原破坏了蛋白质的这种功能。Kabi纤维蛋白原的高溶解度级分，片段X（阶段1）和芳香剂X（阶段2），与完整的纤维蛋白原相比，在促进ADP诱导的血小板聚集方面的效力分别低2倍、8倍和10倍。片段Y和D以及人纤维蛋白原的还原链和羧甲基化链的混合物根本不支持ADP诱导的血小板聚集。非还原和还原纤维蛋白原及其衍生物的SDS聚丙烯酰胺凝胶电泳表明，完整的纤维蛋白原分子是ADP诱导的血小板聚集所必需的。这表明，纤维蛋白原的血小板聚集促进功能所需的A α链的羧基末端部分，也可能是B β链的氨基末端部分。
The presence of human fibrinogen in suspensions of washed human platelets is a requirement for ADP-induced platelet aggregation. Digestion of fibrinogen with plasmin destroys this function of the protein. The high solubility fraction of Kabi fibrinogen, fragment X (stage 1) and framgent X (stage 2), are two, eight, and ten times, respectively, less potent in promoting ADP-induced platelet aggregation, as compared with intact fibrinogen. Fragments Y and D and the mixture of reduced and carboxymethylated chains of human fibrinogen do not support ADP-induced platelet aggregation at all. SDS polyacrylamide gel electrophoresis of nonreduced and reduced fibrinogen and its derivatives indicates that the intact fibrinogen molecule is essential for ADP-induced platelet aggregation. It is suggested that the carboxy-terminal part of the Aalpha chain and possibly also the amino-terminal part of the Bbeta chain are required for the platelet aggregation-promoting function of fibrinogen.