Activin incorporation into vitellogenic oocytes of Xenopus laevis.
Activin incorporation into vitellogenic oocytes of Xenopus laevis.
复制标题
激活素掺入非洲爪蟾的卵黄发生卵母细胞中。
DOI：
--
复制
发表时间：
1999
影响因子：
1.6
通讯作者：
M. Asashima
中科院分区：
文献类型：
--
作者：
A. Fukui;R. Shiurba;M. Asashima
激活素摄取到非洲爪蟾卵母细胞研究了几个互补的方法。免疫细胞化学显示激活素和卵泡抑素主要分布于卵黄发生卵母细胞和卵泡周围细胞的胞浆中。蛋白质相互作用动力学的表面等离子体共振分析表明，而卵泡抑素或激活素卵泡抑素复合物结合到蛋黄卵黄蛋白原，单独激活素没有。放射性示踪剂分析测量体外活卵母细胞激活素的特异性掺入。总之，结果表明，卵黄发生卵母细胞可以进口激活素从卵泡细胞和卵泡抑素可能作为伴侣结合激活素卵黄血小板中的卵黄蛋白原。
Activin uptake into Xenopus oocytes was studied by several complementary methods. Immunocytochemistry of adult ovary localized activin and follistatin in the cytoplasm of vitellogenic oocytes and surrounding follicle cells. Surface plasmon resonance analysis of protein interaction kinetics indicated that while follistatin or a complex of activin-follistatin bound to yolk vitellogenin, activin alone did not. Radioactive tracer analysis measured specific incorporation of activin by viable oocytes in vitro. Together, the results suggest that vitellogenic oocytes can import activins from follicle cells and that follistatin may act as a chaperone for binding activin to vitellogenin in yolk platelets.