Biosynthetic Origin of Formylaminooxyvinylglycine and Characterization of the Formyltransferase GvgI
Biosynthetic Origin of Formylaminooxyvinylglycine and Characterization of the Formyltransferase GvgI
复制标题
甲酰氨基氧基乙烯基甘氨酸的生物合成来源和甲酰转移酶 GvgI 的表征
DOI：
10.1021/acs.biochem.2c00374
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发表时间：
2022
期刊：
影响因子：
2.9
通讯作者：
Li, Bo
中科院分区：
文献类型：
--
作者：
Lescallette, Adam R.;Dunn, Zachary D.;Manning, Viola A.;Trippe, Kristin M.;Li, Bo
4-Formylaminooxyvinylglycine (FVG) is an herbicidal and antibacterial nonproteinogenic amino acid produced by several strains of thePseudomonas fluorescensspecies complex. It contains a unique vinyl alkoxyamine moiety with an O–N bond, and its biosynthetic origin remains unknown. Here, we show that thegvgcluster fromP. fluorescensWH6 is responsible for the biosynthesis of FVG and two additional O–N bond-containing oxyvinylglycines, guanidinooxyvinylglycine and aminooxyvinylglycine. Feeding studies in the producing bacteria indicate that these compounds originate from homoserine. We identify a formyltransferasegvgIthat is required for the production of FVG and characterize the activity of this enzymein vitrotoward amino acids with a side chain amine. Sequence similarity network analysis reveals that GvgI and homologues make up a distinct group from the main classes of formyltransferases.
