Steady increase in cellular HIV-1 load during the asymptomatic phase of untreated infection despite stable plasma viremia
Steady increase in cellular HIV-1 load during the asymptomatic phase of untreated infection despite stable plasma viremia
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DOI：
10.1097/qad.0b013e32833b3171
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发表时间：
2010-07-01
期刊：
影响因子：
3.8
通讯作者：
Lukashov, Vladimir V.
中科院分区：
文献类型：
--
作者：
Pasternak, Alexander O.;Jurriaans, Suzanne;Lukashov, Vladimir V.
目的：比较未经治疗的HIV - 1感染无症状期外周血单个核细胞（PBMCs）和血浆中HIV - 1分子标志物的动态变化。
设计与方法：采用半巢式实时聚合酶链反应（PCR）检测方法，我们在10名未经治疗的HIV - 1感染男性无症状感染期的多个时间点测量了其外周血单个核细胞中HIV - 1前病毒（pr）DNA、未剪接（us）RNA和多重剪接RNA的水平，并将这些标志物的纵向趋势与血浆中病毒RNA的趋势进行了比较。
结果：尽管任何个体的血浆RNA水平均无显著变化，但10人中有6人的未剪接RNA水平随时间显著升高，4人的前病毒DNA水平升高。未剪接RNA、前病毒DNA和CD4⁺细胞计数（而非血浆RNA）从基线的斜率、变化和时间加权变化均显著不同于零（P
Objective: To compare the dynamics of HIV-1 molecular markers in peripheral blood mononuclear cells (PBMCs) and in plasma during the asymptomatic phase of untreated HIV-1 infection.Design and methods: Using seminested real-time PCR assays, we measured the levels of HIV-1 proviral (pr) DNA, unspliced (us) RNA, and multiply spliced RNA in the PBMCs of 10 untreated HIV-1-infected men at multiple time points during the asymptomatic phase of infection and compared the longitudinal trends of these markers with those of viral RNA in plasma.Results: Whereas plasma RNA levels did not significantly change in any of the individuals, levels of usRNA significantly increased with time in six out of 10 persons, and levels of prDNA in four. Slopes, changes, and time-weighted changes from baseline of usRNA, prDNA, and CD4(+) cell count, but not of plasma RNA, were significantly different from zero (P