A novel cobalt complex for enhancing amperometric and impedimetric DNA detection
A novel cobalt complex for enhancing amperometric and impedimetric DNA detection
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DOI：
10.1016/j.electacta.2013.10.028
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发表时间：
2014-05
影响因子：
6.6
通讯作者：
E. Regan;A. Hallett;L. Wong;Ibrahim Q. Saeed;Emily E. Langdon-Jones;N. Buurma;Simon J. A. Pope;P. Estrela
中科院分区：
文献类型：
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作者：
E. Regan;A. Hallett;L. Wong;Ibrahim Q. Saeed;Emily E. Langdon-Jones;N. Buurma;Simon J. A. Pope;P. Estrela
在这项工作中，我们提出了一种新的钴配合物，[Co（GA）2（aqphen）]Cl，是水溶性的，氧化还原活性，并结合到dsDNA。我们报告说，这种复合物可以被用作信号增强剂时，检测DNA杂交使用电化学阻抗谱（EIS）和差分脉冲伏安法（DPV）。当与dsDNA结合时，该化合物通过引起电荷转移电阻（Rct）的增加来介导其EIS信号增强。与ssDNA相比，当化合物与dsDNA一起孵育时，也观察到DPV的峰电流增加。我们认为，该化合物特异性地嵌入dsDNA并改变DNA结构，以影响溶液中带电氧化还原标记物的静电屏障。据我们所知，这是第一个例子，一个单一的化合物，可以提高电流和阻抗信号的DNA检测。我们的研究结果使无标记和多模式方法的发展，以提高电化学DNA检测的灵敏度，准确性和速度。
In this work we present a novel cobalt complex, [Co(GA)2(aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (Rct) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection. Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.